ArticleProceedings of the National Academy of Sciences of the United States of America2003
The Escherichia coli multidrug transporter MdfA catalyzes both electrogenic and electroneutral transport reactions.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 93 citations in OpenAlex.
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- Energetic and structural control of polyspecificity in a multidrug transporter.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Simultaneous detection of pathogens and antimicrobial resistance genes with the open source, cloud-based, CZ ID platform.Genome medicine · 2025Article
- Understanding, inhibiting, and engineering membrane transporters with high-throughput mutational screens.Cell chemical biology · 2025Review
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- Dissemination of ResistantFrontiers in microbiology · 2022Article
- Drug resistance: from bacteria to cancer.Molecular biomedicine · 2021Review
- Structures and General Transport Mechanisms by the Major Facilitator Superfamily (MFS).Chemical reviews · 2021Article
- Genetic but No Phenotypic Associations between Biocide Tolerance and Antibiotic Resistance inMicroorganisms · 2021Article
- Drug Permeation against Efflux by Two Transporters.ACS infectious diseases · 2020Article
- Structure and mechanism of a redesigned multidrug transporter from the Major Facilitator Superfamily.Scientific reports · 2020Article
- Impacts of florfenicol on the microbiota landscape and resistome as revealed by metagenomic analysis.Microbiome · 2019Article
- smFRET Probing Reveals Substrate-Dependent Conformational Dynamics of E. coli Multidrug MdfA.Biophysical journal · 2019Article
- Reversing resistance to counter antimicrobial resistance in the World Health Organisation's critical priority of most dangerous pathogens.Bioscience reports · 2019Review
- Structure of an engineered multidrug transporter MdfA reveals the molecular basis for substrate recognition.Communications biology · 2019Article
- Outward open conformation of a Major Facilitator Superfamily multidrug/HNature communications · 2018Article
- The prototypical proton-coupled oligopeptide transporter YdgR fromThe Journal of biological chemistry · 2018Article
- Mrp Antiporters Have Important Roles in Diverse Bacteria and Archaea.Frontiers in microbiology · 2017Review
- Article
- Modeling the Overproduction of Ribosomes when Antibacterial Drugs Act on Cells.Biophysical journal · 2016Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The resistance of cells to many drugs simultaneously (multidrug resistance) often involves the expression of membrane transporters (Mdrs); each recognizes and expels a broad spectrum of chemically unrelated drugs from the cell. The Escherichia coli Mdr transporter MdfA is able to transport differentially charged substrates in exchange for protons. This includes neutral compounds, namely chloramphenicol and thiamphenicol, and lipophilic cations such as tetraphenylphosphonium and ethidium. Here we show that the chloramphenicol and thiamphenicol transport reactions are electrogenic, whereas the transport of several monovalent cationic substrates is electroneutral. Therefore, unlike with positively charged substrates, the transmembrane electrical potential (negative inside) constitutes a major part of the driving force for the transport of electroneutral substrates by MdfA. These results demonstrate an unprecedented ability of a single secondary transporter to catalyze discrete transport reactions that differ in their electrogenicity and are governed by different components of the proton motive force.
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