ArticleBiochemistry2003
Structural basis for a lethal mutation in U6 RNA.
Article in Biochemistry, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- Thermodynamic examination of pH and magnesium effect on U6 RNA internal loop.RNA (New York, N.Y.) · 2019Article
- The life of U6 small nuclear RNA, from cradle to grave.RNA (New York, N.Y.) · 2018Review
- Lights, camera, action! Capturing the spliceosome and pre-mRNA splicing with single-molecule fluorescence microscopy.Wiley interdisciplinary reviews. RNA · 2016Review
- Structural requirements for protein-catalyzed annealing of U4 and U6 RNAs during di-snRNP assembly.Nucleic acids research · 2016Article
- Structural features of a 3' splice site in influenza a.Biochemistry · 2015Article
- Determination of the secondary structure of group II bulge loops using the fluorescent probe 2-aminopurine.RNA (New York, N.Y.) · 2015Article
- Structure of the yeast U2/U6 snRNA complex.RNA (New York, N.Y.) · 2012Article
- Single-molecule analysis of protein-free U2-U6 snRNAs.Nature structural & molecular biology · 2009Article
- Minimum-energy path for a u6 RNA conformational change involving protonation, base-pair rearrangement and base flipping.Journal of molecular biology · 2009Article
- Computational identification of four spliceosomal snRNAs from the deep-branching eukaryote Giardia intestinalis.PloS one · 2008Article
- Guanidinoneomycin B recognition of an HIV-1 RNA helix.Chembiochem : a European journal of chemical biology · 2008Article
- A dynamic bulge in the U6 RNA internal stem-loop functions in spliceosome assembly and activation.RNA (New York, N.Y.) · 2007Article
- Prediction of molecular alignment of nucleic acids in aligned media.Journal of biomolecular NMR · 2006Article
- Consecutive GA pairs stabilize medium-size RNA internal loops.Biochemistry · 2006Article
- Structure of the Tetrahymena thermophila telomerase RNA helix II template boundary element.Nucleic acids research · 2006Article
- The N- and C-terminal RNA recognition motifs of splicing factor Prp24 have distinct functions in U6 RNA binding.RNA (New York, N.Y.) · 2005Article
- Solution structure of the HIV-1 frameshift inducing stem-loop RNA.Nucleic acids research · 2003Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
U6 RNA is essential for nuclear pre-mRNA splicing and has been implicated directly in catalysis of intron removal. The U80G mutation at the essential magnesium binding site of the U6 3' intramolecular stem-loop region (ISL) is lethal in yeast. To further understand the structure and function of the U6 ISL, we have investigated the structural basis for the lethal U80G mutation by NMR and optical spectroscopy. The NMR structure reveals that the U80G mutation causes a structural rearrangement within the ISL resulting in the formation of a new Watson-Crick base pair (C67 x G80), and disrupts a protonated C67 x A79 wobble pair that forms in the wild-type structure. Despite the structural change, the accessibility of the metal binding site is unperturbed, and cadmium titration produces similar phosphorus chemical shift changes for both the U80G mutant and wild-type RNAs. The thermodynamic stability of the U80G mutant is significantly increased (Delta Delta G(fold) = -3.6 +/- 1.9 kcal/mol), consistent with formation of the Watson-Crick pair. Our structural and thermodynamic data, in combination with previous genetic data, suggest that the lethal basis for the U80G mutation is stem-loop hyperstabilization. This hyperstabilization may prevent the U6 ISL melting and rearrangement necessary for association with U4.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.