ArticleProceedings of the National Academy of Sciences of the United States of America2003
Cloning, expression, and characterization of a membrane progestin receptor and evidence it is an intermediary in meiotic maturation of fish oocytes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 236 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
236 citing papers in PubMed, 1 synthesis or guideline pooled it, 793 citations in OpenAlex.
- Vaginal progesterone in women with an asymptomatic sonographic short cervix in the midtrimester decreases preterm delivery and neonatal morbidity: a systematic review and metaanalysis of individual patient data.American journal of obstetrics and gynecology · 2012Pooled it
- CAF-derived PTGDS drives pancreatic cancer neuroendocrine differentiation and chemoresistance via PAQR9-MAPK.Oncogenesis · 2026Article
- Deciphering the miRNA-TF-mRNA Regulatory Network Underlying Oocyte Maturation in Orange-Spotted Grouper (Animals : an open access journal from MDPI · 2026Article
- Review
- Environmental and Physiological Regulation of Reproduction in the Goldfish: Gonadal Development, Maturation, and Spawning Behavior: A Review.Animals : an open access journal from MDPI · 2026Review
- ChIP happens: from biochemical origins to the modern omics toolbox for understanding steroid hormone receptors.The Biochemical journal · 2026Review
- Identification of a novel natural compound that acts on the membrane progestin receptor α (paqr7) from the marine algae Padina.Scientific reports · 2026Article
- Progesterone signaling in cardio-renal physiology and pathophysiology.Frontiers in endocrinology · 2026Review
- Article
- Effects of progestogen neurosteroids on locomotor activity in zebrafish embryos and larvae.Fish physiology and biochemistry · 2025Article
- Progesterone Enhances Sensitivity of Ovarian Cancer Cells to SN38 Through Inhibition of Topoisomerase I and Inducing Ferroptosis.Cancer reports (Hoboken, N.J.) · 2025Article
- Hormonal dynamics reveal a stimulatory role for secretoneurin in zebrafish ovulation.PNAS nexus · 2025Article
- Amphibians and their impact on human reproduction research.F&S reports · 2025Article
- Article
- Article
- Transcriptomic Response of the Ovarian Follicle Complex in Post-Vitellogenic Rainbow Trout to 17α,20β-Dihdroxy-4-pregnen-3-one In Vitro.International journal of molecular sciences · 2024Article
- Progesterone induces meiosis through two obligate co-receptors with PLA2 activity.bioRxiv : the preprint server for biology · 2024Article
- Establishment of a graphene quantum dot (GQD) based steroid binding assay for the nuclear progesterone receptor (pgr).Biochemistry and biophysics reports · 2024Article
- Establishment of a steroid binding assay for goldfish membrane progesterone receptor (mPR) by coupling with graphene quantum dots (GQDs).Fish physiology and biochemistry · 2024Article
- Deciphering the function of the fifth class of Gα proteins: regulation of ionic homeostasis as unifying hypothesis.Cellular and molecular life sciences : CMLS · 2024Article
176 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The structures of membrane receptors mediating rapid, nongenomic actions of steroids have not been identified. We describe the cloning of a cDNA from spotted seatrout ovaries encoding a protein that satisfies the following seven criteria for its designation as a steroid membrane receptor: plausible structure, tissue specificity, cellular distribution, steroid binding, signal transduction, hormonal regulation, and biological relevance. For plausible structure, computer modeling predicts that the protein has seven transmembrane domains, typical of G protein-coupled receptors. The mRNA (4.0 kb) is only detected in the brain and reproductive tissues on Northern blots. Antisera only detect the protein (40 kDa) in plasma membranes of reproductive tissues. The recombinant protein produced in an Escherichia coli expression system has a high affinity (K(d) = 30 nM), saturable, displaceable, single binding site specific for progestins. Progestins alter signal transduction pathways, activating mitogen-activated protein kinase and inhibiting adenylyl cyclase, in a transfected mammalian cell line. Inhibition of adenylyl cyclase is pertussis toxin sensitive, suggesting the receptor may be coupled to an inhibitory G protein. Progestins and gonadotropin up-regulate both mRNA and protein levels in seatrout ovaries. Changes in receptor abundance in response to hormones and at various stages of oocyte development, its probable coupling to an inhibitory G protein and inhibition of progestin induction of oocyte maturation upon microinjection of antisense oligonucleotides are consistent with the identity of the receptor as an intermediary in oocyte maturation. These characteristics suggest the fish protein is a membrane progestin receptor mediating a "nonclassical" action of progestins to induce oocyte maturation in fish.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.