ArticleJournal of clinical microbiology2003
Multilocus sequence typing reveals a lack of diversity among Escherichia coli O157:H7 isolates that are distinct by pulsed-field gel electrophoresis.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed.
- The Microbiome of Fertilization-Stage Maize Silks (Style) Encodes Genes and Expresses Traits That Potentially Promote Survival in Pollen/Style Niches and Host Reproduction.Microorganisms · 2024Article
- Whole-Genome Sequencing of Shiga Toxin-ProducingMicroorganisms · 2023Article
- Review ofInfection and drug resistance · 2022Review
- Identification and detection of pathogenic bacteria from patients with hospital-acquired pneumonia in southwestern Iran; evaluation of biofilm production and molecular typing of bacterial isolates.BMC pulmonary medicine · 2021Article
- Escherichia coli-associated granulomatous colitis in dogs treated according to antimicrobial susceptibility profiling.Journal of veterinary internal medicine · 2021Article
- Prevalence and Genetic Characterization ofJournal of microbiology and biotechnology · 2020Article
- Genetic Diversity of Non-O157 Shiga Toxin-ProducingFrontiers in microbiology · 2020Article
- Laboratory Methods in Molecular Epidemiology: Bacterial Infections.Microbiology spectrum · 2018Review
- Isolation, Identification, Prevalence, and Genetic Diversity ofFrontiers in microbiology · 2018Article
- Recent Advances in Molecular Diagnosis ofFrontiers in microbiology · 2018Article
- Characteristics and genetic diversity of multi-drug resistant extended-spectrum beta-lactamase (ESBL)-producingOncotarget · 2017Article
- Characterization of Shiga toxin-producing Escherichia coli from feces of sika deer (Cervus nippon) in Japan using PCR binary typing analysis to evaluate their potential human pathogenicity.The Journal of veterinary medical science · 2017Article
- Culture independent analysis using gnd as a target gene to assess Escherichia coli diversity and community structure.Scientific reports · 2017Article
- Article
- A combinational approach of multilocus sequence typing and other molecular typing methods in unravelling the epidemiology of Erysipelothrix rhusiopathiae strains from poultry and mammals.Veterinary research · 2015Article
- Clade 8 and Clade 6 Strains of Escherichia coli O157:H7 from Cattle in Argentina have Hypervirulent-Like Phenotypes.PloS one · 2015Article
- Phylogenetic Clades 6 and 8 of Enterohemorrhagic Escherichia coli O157:H7 With Particular stx Subtypes are More Frequently Found in Isolates From Hemolytic Uremic Syndrome Patients Than From Asymptomatic Carriers.Open forum infectious diseases · 2014Article
- Prevalence and characteristics of Shiga toxin-producing Escherichia coli (STEC) from cattle in Korea between 2010 and 2011.Journal of veterinary science · 2014Article
- Molecular characterization of enterohemorrhagic E. coli O157 isolated from animal fecal and food samples in Eastern China.TheScientificWorldJournal · 2014Article
- Development and application of MLVA methods as a tool for inter-laboratory surveillance.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2013Review
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7 authors.
Funding
Abstract
Escherichia coli O157:H7 is a major cause of foodborne illness in the United States. Pulsed-field gel electrophoresis (PFGE) is the molecular epidemiologic method mostly commonly used to identify food-borne outbreaks. Although PFGE is a powerful epidemiologic tool, it has disadvantages that make a DNA sequence-based approach potentially attractive. Multilocus sequence typing (MLST) analyzes the internal fragments of housekeeping genes to establish genetic relatedness between isolates. We sequenced selected portions of seven housekeeping genes and two membrane protein genes (ompA and espA) of 77 isolates that were diverse by PFGE to determine whether there was sufficient sequence variation to be useful as an epidemiologic tool. There was no DNA sequence diversity in the sequenced portions of the seven housekeeping genes and espA. For ompA, all but five isolates had sequence identical to that of the reference strains. E. coli O157:H7 has a striking lack of genetic diversity in the genes we explored, even among isolates that are clearly distinct by PFGE. Other approaches to identify improved molecular subtyping methods for E. coli 0157:H7 are needed.
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