ArticleJournal of clinical microbiology2003
Mixed-genome microarrays reveal multiple serotype and lineage-specific differences among strains of Listeria monocytogenes.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 118 citations in OpenAlex.
- Is the persistence ofApplied and environmental microbiology · 2024Article
- Novel Approaches to Environmental Monitoring and Control ofFoods (Basel, Switzerland) · 2022Review
- Whole genome genetic variation and linkage disequilibrium in a diverse collection of Listeria monocytogenes isolates.PloS one · 2021Article
- Genotypes Associated withFrontiers in microbiology · 2017Article
- Genomic characterization of novel Listeria monocytogenes serotype 4b variant strains.PloS one · 2014Article
- Formation of biofilm by Listeria monocytogenes ATCC 19112 at different incubation temperatures and concentrations of sodium chloride.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2013Article
- Salmonella Enteritidis strains from poultry exhibit differential responses to acid stress, oxidative stress, and survival in the egg albumen.Foodborne pathogens and disease · 2012Article
- Article
- Recombination and positive selection contributed to the evolution of Listeria monocytogenes lineages III and IV, two distinct and well supported uncommon L. monocytogenes lineages.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2011Article
- Gene Scanning of an Internalin B Gene Fragment Using High-Resolution Melting Curve Analysis as a Tool for Rapid Typing of Listeria monocytogenes.The Journal of molecular diagnostics : JMD · 2011Article
- Probing the pan-genome of Listeria monocytogenes: new insights into intraspecific niche expansion and genomic diversification.BMC genomics · 2010Article
- Efficient oligonucleotide probe selection for pan-genomic tiling arrays.BMC bioinformatics · 2009Article
- Comparative proteomic analysis of Listeria monocytogenes strains F2365 and EGD.Applied and environmental microbiology · 2009Article
- Rapid identification and typing of listeria species by matrix-assisted laser desorption ionization-time of flight mass spectrometry.Applied and environmental microbiology · 2008Article
- Lineage specific recombination and positive selection in coding and intragenic regions contributed to evolution of the main Listeria monocytogenes virulence gene cluster.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2008Article
- A Java-based tool for the design of classification microarrays.BMC bioinformatics · 2008Article
- Comparative genomics of "Dehalococcoides ethenogenes" 195 and an enrichment culture containing unsequenced "Dehalococcoides" strains.Applied and environmental microbiology · 2008Article
- Comparative transcriptome analysis of Listeria monocytogenes strains of the two major lineages reveals differences in virulence, cell wall, and stress response.Applied and environmental microbiology · 2007Article
- Validation of mixed-genome microarrays as a method for genetic discrimination.Applied and environmental microbiology · 2007Article
- A perspective on microarrays: current applications, pitfalls, and potential uses.Microbial cell factories · 2007Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Epidemiological studies and analysis of putative virulence genes have shown that Listeria monocytogenes has diverged into several phylogenetic divisions. We hypothesize that similar divergence has occurred for many genes that influence niche-specific fitness and virulence and that identifying these differences may offer new opportunities for the detection, treatment, and control of this important pathogen. To explore this issue further, we developed a microarray composed of fragmented DNA taken from 10 strains of L. monocytogenes. We then hybridized genomic DNA from 50 different strains to replicate arrays and analyzed the resulting hybridization patterns. A simple Euclidean distance metric permitted the reconstruction of previously described genetic relationships between serotypes, and only four microarray probes were needed to discriminate between the most important serotypes (1/2a, 1/2b, 1/2c, and 4). We calculated an index of linkage equilibrium from the microarray data and confirmed that L. monocytogenes has a strongly clonal population structure (I(A) = 3.85). Twenty-nine informative probes were retrieved from the library and sequenced. These included genes associated with repairing UV-damaged DNA, salt tolerance, biofilm formation, heavy metal transport, ferrous iron transport, and teichoic acid synthesis. Several membrane-bound lipoproteins and one internalin were identified, plus three phage sequences and six sequences with unknown function. Collectively, these data confirm that many genes have diverged between lineages of L. monocytogenes. Furthermore, these results demonstrate the value of mixed-genome microarrays as a tool for deriving biologically useful information and for identifying and screening genetic markers for clinically important microbes.
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