ArticleJournal of clinical microbiology2003
Highly polymorphic microsatellite for identification of Candida albicans strains.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 105 citations in OpenAlex.
- Migration and standing variation in vaginal and rectal yeast populations in recurrent vulvovaginal candidiasis.mSystems · 2025Article
- Detection of Multiple Nosocomial Trichosporon asahii Transmission Events via Microsatellite Typing Assay, South America.Emerging infectious diseases · 2025Article
- Typing of feces-derived Candida albicans strains using a novel seven-locus microsatellite panel reveals associations with yeast phenotype in individuals with inflammatory bowel disease.Pathogens and disease · 2025Article
- Short Tandem Repeat Genotyping of Medically Important Fungi: A Comprehensive Review of a Powerful Tool with Extensive Future Potential.Mycopathologia · 2024Review
- Novel Identification of the Collection of Pathogenic Fungal SpeciesPathogens (Basel, Switzerland) · 2023Article
- Vertical Transmission and Antifungal Susceptibility Profile of Yeast Isolates from the Oral Cavity, Gut, and Breastmilk of Mother-Child Pairs in Early Life.International journal of molecular sciences · 2023Article
- Biased Genotype Distributions of Candida albicans Strains Associated with 649 Clinical Vulvovaginal Candidiasis in China.Mycopathologia · 2022Article
- Phenotype, genotype, and mating type determination in oral Candida albicans isolates from pediatric patients with neutropenia.Journal of clinical laboratory analysis · 2022Article
- OralJournal of oral microbiology · 2020Article
- Genotyping Reveals High Clonal Diversity and Widespread Genotypes ofFrontiers in cellular and infection microbiology · 2020Article
- Virulence and antifungal susceptibility of microsatellite genotypes of Candida albicans from superficial and deep locations.Yeast (Chichester, England) · 2019Article
- Genetic diversity and antifungal susceptibility of Candida albicans isolated from Iranian patients.Medical mycology · 2019Article
- Relevance of Macrophage Extracellular Traps inFrontiers in immunology · 2019Article
- Reduction in Percentage of Clusters of Candida albicans and Candida parapsilosis Causing Candidemia in a General Hospital in Madrid, Spain.Journal of clinical microbiology · 2018Article
- Genetic Differentiation, Diversity, and Drug Susceptibility ofFrontiers in microbiology · 2018Article
- Investigating Clinical Issues by Genotyping of Medically Important Fungi: Why and How?Clinical microbiology reviews · 2017Review
- The Correlation Between Candida Colonization of Distinct Body Sites and Invasive Candidiasis in Emergency Intensive Care Units: Statistical and Molecular Biological Analysis.Mycopathologia · 2016Article
- Phenotypic diversity and correlation between white-opaque switching and the CAI microsatellite locus in Candida albicans.Current genetics · 2016Article
- Progresses in vaginal microflora physiology and implications for bacterial vaginosis and candidiasis.Women's health (London, England) · 2016Article
- Genetic variability of Candida albicans Sap8 propeptide in isolates from different types of infection.BioMed research international · 2015Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The polymorphism of a new microsatellite locus (CAI) was investigated in a total of 114 Candida albicans strains, including 73 independent clinical isolates, multiple isolates from the same patient, isolates from several episodes of recurrent vulvovaginal infections, and two reference strains. PCR genotyping was performed automatically, using a fluorescence-labeled primer, and in the 73 independent isolates, 26 alleles and 44 different genotypes were identified, resulting in a discriminatory power of 0.97. CAI was revealed to be species specific and showed a low mutation rate, since no amplification product was obtained when testing other pathogenic Candida species and no genotype differences were observed when testing over 300 generations. When applying this microsatellite to the identification of strains isolated from recurrent vulvovaginal infections in eight patients, it was found that 13 out of 15 episodes were due to the same strain. When multiple isolates, obtained from the same patient and plated simultaneously, were typed for CAI, the same genotype was found in each case, confirming that the infecting population was clonal. Moreover, the same genotype appeared in isolates from the rectum and the vagina, revealing that the former could be a reservoir of potentially pathogenic strains. This new microsatellite proves to be a valuable tool to differentiate C. albicans strains. Furthermore, when compared to other molecular genotyping techniques, CAI proved to be very simple, highly efficient, and reproducible, being suitable for low-quantity and very-degraded samples and for application in large-scale epidemiological studies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.