ArticleThe EMBO journal2003
Multiple ORC-binding sites are required for efficient MCM loading and origin firing in fission yeast.
Article in The EMBO journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 53 citations in OpenAlex.
- DNA replication timing: Biochemical mechanisms and biological significance.BioEssays : news and reviews in molecular, cellular and developmental biology · 2022Article
- A predictable conserved DNA base composition signature defines human core DNA replication origins.Nature communications · 2020Article
- Bioinformatical dissection of fission yeast DNA replication origins.Open biology · 2020Article
- Bidirectional eukaryotic DNA replication is established by quasi-symmetrical helicase loading.Science (New York, N.Y.) · 2017Article
- Sap1 is a replication-initiation factor essential for the assembly of pre-replicative complex in the fission yeastThe Journal of biological chemistry · 2017Article
- Prereplication-complex formation: a molecular double take?Nature structural & molecular biology · 2014Article
- Sequence analysis of origins of replication in the Saccharomyces cerevisiae genomes.Frontiers in microbiology · 2014Article
- Telomere-binding protein Taz1 controls global replication timing through its localization near late replication origins in fission yeast.Genes & development · 2012Article
- Chromatin architectures at fission yeast transcriptional promoters and replication origins.Nucleic acids research · 2012Article
- Altered replication in human cells promotes DMPK (CTG)(n) · (CAG)(n) repeat instability.Molecular and cellular biology · 2012Article
- Nucleosomal organization of replication origins and meiotic recombination hotspots in fission yeast.The EMBO journal · 2012Article
- The origin recognition complex: a biochemical and structural view.Sub-cellular biochemistry · 2012Review
- Analysis of model replication origins in Drosophila reveals new aspects of the chromatin landscape and its relationship to origin activity and the prereplicative complex.Molecular biology of the cell · 2012Article
- Abundance of prereplicative complexes (Pre-RCs) facilitates recombinational repair under replication stress in fission yeast.The Journal of biological chemistry · 2011Article
- Regulation of DNA replication by chromatin structures: accessibility and recruitment.Chromosoma · 2011Review
- Structural diversity and dynamics of genomic replication origins in Schizosaccharomyces pombe.The EMBO journal · 2010Article
- Acetylation by GCN5 regulates CDC6 phosphorylation in the S phase of the cell cycle.Nature structural & molecular biology · 2009Article
- Article
- Genome-wide localization of pre-RC sites and identification of replication origins in fission yeast.The EMBO journal · 2007Article
- DNA elements modulating the KARS12 chromosomal replicator in Kluyveromyces lactis.Molecular genetics and genomics : MGG · 2007Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In most eukaryotes, replication origins are composed of long chromosome regions, and the exact sequences required for origin recognition complex (ORC) and minichromosome maintenance (MCM) complex association remain elusive. Here, we show that two stretches of adenine/thymine residues are collectively essential for a fission yeast chromosomal origin. Chromatin immunoprecipitation assays revealed that the ORC subunits are located within a 1 kb region of ori2004. Analyses of deletion derivatives of ori2004 showed that adenine stretches are required for ORC binding in vivo. Synergistic interaction between ORC and adenine stretches was observed. On the other hand, MCM subunits were localized preferentially to a region near the initiation site, which is distant from adenine stretches. This association was dependent on adenine stretches and stimulated by a non-adenine element. Our results suggest that association of multiple ORC molecules with a replication origin is required for efficient MCM loading and origin firing in fission yeast.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.