ArticleThe EMBO journal2003
E1 initiator DNA binding specificity is unmasked by selective inhibition of non-specific DNA binding.
Article in The EMBO journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 48 citations in OpenAlex.
- A conserved regulatory module at the C terminus of the papillomavirus E1 helicase domain controls E1 helicase assembly.Journal of virology · 2015Article
- The papillomavirus E2 proteins.Virology · 2013Review
- The E1 proteins.Virology · 2013Review
- CK2 phosphorylation inactivates DNA binding by the papillomavirus E1 and E2 proteins.Journal of virology · 2013Article
- Nuclear accumulation of the papillomavirus E1 helicase blocks S-phase progression and triggers an ATM-dependent DNA damage response.Journal of virology · 2011Article
- The inhibitory action of P56 on select functions of E1 mediates interferon's effect on human papillomavirus DNA replication.Journal of virology · 2010Article
- Effective formation of the segregation-competent complex determines successful partitioning of the bovine papillomavirus genome during cell division.Journal of virology · 2010Article
- Interaction of the papillomavirus E8--E2C protein with the cellular CHD6 protein contributes to transcriptional repression.Journal of virology · 2010Article
- Structure-based mutational analysis of the bovine papillomavirus E1 helicase domain identifies residues involved in the nonspecific DNA binding activity required for double trimer formation.Journal of virology · 2010Article
- A non-sequence-specific DNA binding mode of RAG1 is inhibited by RAG2.Journal of molecular biology · 2009Article
- Functional mapping of the human papillomavirus type 16 E1 cistron.Journal of virology · 2008Article
- A DNA-binding activity in BPV initiator protein E1 required for melting duplex ori DNA but not processive helicase activity initiated on partially single-stranded DNA.Nucleic acids research · 2008Article
- Human papillomavirus E1 helicase interacts with the WD repeat protein p80 to promote maintenance of the viral genome in keratinocytes.Journal of virology · 2008Article
- Replication and partitioning of papillomavirus genomes.Advances in virus research · 2008Review
- ATP-dependent minor groove recognition of TA base pairs is required for template melting by the E1 initiator protein.Journal of virology · 2007Article
- Dynamic localization of the human papillomavirus type 11 origin binding protein E2 through mitosis while in association with the spindle apparatus.Journal of virology · 2006Article
- Cellular topoisomerase I modulates origin binding by bovine papillomavirus type 1 E1.Journal of virology · 2006Article
- Papillomavirus E1 protein binds to and stimulates human topoisomerase I.Journal of virology · 2006Article
- Common determinants in DNA melting and helicase-catalysed DNA unwinding by papillomavirus replication protein E1.Nucleic acids research · 2006Article
- The X-ray structure of the papillomavirus helicase in complex with its molecular matchmaker E2.Genes & development · 2004Article
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Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
Initiator proteins are critical components of the DNA replication machinery and mark the site of initiation. This activity probably requires highly selective DNA binding; however, many initiators display modest specificity in vitro. We demonstrate that low specificity of the papillomavirus E1 initiator results from the presence of a non-specific DNA-binding activity, involved in melting, which masks the specificity intrinsic to the E1 DNA-binding domain. The viral factor E2 restores specificity through a physical interaction with E1 that suppresses non-specific binding. We propose that this arrangement, where one DNA-binding activity tethers the initiator to ori while another alters DNA structure, is a characteristic of other viral and cellular initiator proteins. This arrangement would provide an explanation for the low selectivity observed for DNA binding by initiator proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.