ArticleThe EMBO journal2003
The C-terminal domain of pol II and a DRB-sensitive kinase are required for 3' processing of U2 snRNA.
Article in The EMBO journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed.
- The Integrator complex: an emerging complex structure involved in the regulation of gene expression by targeting RNA polymerase II.Functional & integrative genomics · 2024Review
- An Important Role for RPRD1B in the Heat Shock Response.Molecular and cellular biology · 2022Article
- CDK9 and PP2A regulate RNA polymerase II transcription termination and coupled RNA maturation.EMBO reports · 2022Article
- CAPTURE of the Human U2 snRNA Genes Expands the Repertoire of Associated Factors.Biomolecules · 2022Article
- Oxidative stress induces Ser 2 dephosphorylation of the RNA polymerase II CTD and premature transcription termination.Transcription · 2021Article
- Diverse and conserved roles of the protein Ssu72 in eukaryotes: from yeast to higher organisms.Current genetics · 2021Review
- The Integrator Complex in Transcription and Development.Trends in biochemical sciences · 2020Review
- Alternative splicing of DSP1 enhances snRNA accumulation by promoting transcription termination and recycle of the processing complex.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Gene-Specific Control of tRNA Expression by RNA Polymerase II.Molecular cell · 2020Article
- Anthralin Suppresses the Proliferation of Influenza Virus by Inhibiting the Cap-Binding and Endonuclease Activity of Viral RNA Polymerase.Frontiers in microbiology · 2020Article
- Salt Stress and CTD PHOSPHATASE-LIKE4 Mediate the Switch between Production of Small Nuclear RNAs and mRNAs.The Plant cell · 2017Article
- The 7SK snRNP associates with the little elongation complex to promote snRNA gene expression.The EMBO journal · 2017Article
- snRNA 3' End Processing by a CPSF73-Containing Complex Essential for Development in Arabidopsis.PLoS biology · 2016Article
- Integrator: surprisingly diverse functions in gene expression.Trends in biochemical sciences · 2015Review
- CDK9 inhibitors define elongation checkpoints at both ends of RNA polymerase II-transcribed genes.Nature structural & molecular biology · 2015Article
- CTCF regulates NELF, DSIF and P-TEFb recruitment during transcription.Transcription · 2015Article
- Function and control of RNA polymerase II C-terminal domain phosphorylation in vertebrate transcription and RNA processing.Molecular and cellular biology · 2014Article
- A role for the Cajal-body-associated SUMO isopeptidase USPL1 in snRNA transcription mediated by RNA polymerase II.Journal of cell science · 2014Article
- Article
- The little elongation complex functions at initiation and elongation phases of snRNA gene transcription.Molecular cell · 2013Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The human snRNA genes transcribed by RNA polymerase II (e.g. U1 and U2) have a characteristic TATA-less promoter containing an essential proximal sequence element. Formation of the 3' end of these non-polyadenylated RNAs requires a specialized 3' box element whose function is promoter specific. Here we show that truncation of the C-terminal domain (CTD) of RNA polymerase II and treatment of cells with CTD kinase inhibitors, including DRB (5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole), causes a dramatic reduction in proper 3' end formation of U2 transcripts. Activation of 3' box recognition by the phosphorylated CTD would be consistent with the role of phospho-CTD in mRNA processing. CTD kinase inhibitors, however, have little effect on initiation or elongation of transcription of the U2 genes, whereas elongation of transcription of the beta-actin gene is severely affected. This result highlights differences in transcription of snRNA and mRNA genes.
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