ArticleThe EMBO journal2003
Thylakoid targeting of Tat passenger proteins shows no delta pH dependence in vivo.
Article in The EMBO journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 69 citations in OpenAlex.
- The ten amino acids of the oxygen-evolving enhancer of tobacco is sufficient as the peptide residues for protein transport to the chloroplast thylakoid.Plant molecular biology · 2021Article
- Transport of Folded Proteins by the Tat System.The protein journal · 2019Review
- The Twin-Arginine Pathway for Protein Secretion.EcoSal Plus · 2019Review
- The Tat protein transport system: intriguing questions and conundrums.FEMS microbiology letters · 2018Review
- Intra-plastid protein trafficking: how plant cells adapted prokaryotic mechanisms to the eukaryotic condition.Biochimica et biophysica acta · 2013Review
- Twin-arginine-dependent translocation of folded proteins.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2012Review
- Chloroplast biogenesis: control of plastid development, protein import, division and inheritance.The arabidopsis book · 2008Article
- Two electrical potential-dependent steps are required for transport by the Escherichia coli Tat machinery.The Journal of cell biology · 2007Article
- The chloroplast Tat pathway utilizes the transmembrane electric potential as an energy source.Biophysical journal · 2007Article
- The bacterial twin-arginine translocation pathway.Annual review of microbiology · 2006Review
- Subcellular localization and light-regulated expression of protoporphyrinogen IX oxidase and ferrochelatase in Chlamydomonas reinhardtii.Plant physiology · 2005Article
- Large-scale translocation reversal within the thylakoid Tat system in vivo.The Journal of cell biology · 2005Article
- Chlamydomonas immunophilins and parvulins: survey and critical assessment of gene models.Eukaryotic cell · 2005Review
- Efficient assembly of photosystem II in Chlamydomonas reinhardtii requires Alb3.1p, a homolog of Arabidopsis ALBINO3.The Plant cell · 2004Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
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Abstract
The Tat pathway is a major route for protein export in prokaryotes and for protein targeting to thylakoids in chloroplasts. Based on in vitro studies, protein translocation through this pathway is thought to be strictly dependent on a transmembrane delta pH. In this paper, we assess the delta pH sensitivity of the Tat pathway in vivo. Using Chlamydomonas reinhardtii, we observed changes in the efficiency of thylakoid targeting in vivo by mutating the Tat signal of the Rieske protein. We then employed two endogenous pH probes located on the lumen side of the thylakoid membranes to estimate spectroscopically the delta pH in vivo. Using experimental conditions in which the trans-thylakoid delta pH was almost zero, we found no evidence for a delta pH dependence of the Tat pathway in vivo. We confirmed this observation in higher plants using attached barley leaves. We conclude that the Tat pathway does not require a delta pH under physiological conditions, but becomes delta pH sensitive when probed in vitro/in organello because of the loss of some critical intracellular factors.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.