ArticleGene therapy2003
Non-invasive gamma camera imaging of gene transfer using an adenoviral vector encoding an epitope-tagged receptor as a reporter.
Article in Gene therapy, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 29 citations in OpenAlex.
- Molecular Imaging with Reporter Genes: Has Its Promise Been Delivered?Journal of nuclear medicine : official publication, Society of Nuclear Medicine · 2019Review
- Review
- Radiolabeling strategies for radionuclide imaging of stem cells.Stem cell reviews and reports · 2015Article
- Adenoviral-mediated imaging of gene transfer using a somatostatin receptor-cytosine deaminase fusion protein.Cancer gene therapy · 2015Article
- The somatostatin analog 188Re-P2045 inhibits the growth of AR42J pancreatic tumor xenografts.Journal of nuclear medicine : official publication, Society of Nuclear Medicine · 2014Article
- An animal model allowing controlled receptor expression for molecular ultrasound imaging.Ultrasound in medicine & biology · 2013Article
- Imaging of cells and nanoparticles: implications for drug delivery to the brain.Pharmaceutical research · 2012Review
- Identification of critical residues involved in ligand binding and G protein signaling in human somatostatin receptor subtype 2.Endocrinology · 2012Article
- Positron emission tomography reporter genes and reporter probes: gene and cell therapy applications.Theranostics · 2012Article
- Noninvasive cell-tracking methods.Nature reviews. Clinical oncology · 2011Review
- Experimental models to study lymphatic and blood vascular metastasis.Journal of surgical oncology · 2011Review
- Noninvasive repetitive imaging of somatostatin receptor 2 gene transfer with positron emission tomography.Human gene therapy · 2011Article
- SSTR2-based reporters for assessing gene transfer into non-small cell lung cancer: evaluation using an intrathoracic mouse model.Human gene therapy · 2011Article
- Multimodality imaging of gene transfer with a receptor-based reporter gene.Journal of nuclear medicine : official publication, Society of Nuclear Medicine · 2010Article
- [Cell tracking. Principles and applications].Der Radiologe · 2007Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
A model epitope-tagged receptor was constructed by fusing the hemagglutinin (HA) sequence on the extracellular N-terminus of the human somatostatin receptor subtype 2 (hSSTr2) gene. This construct was placed in an adenoviral (Ad-HAhSSTr2) vector. This study evaluated Ad-HAhSSTr2 in vitro and in vivo using FACS, fluorescent microscopy, radioactive binding assays, and gamma camera imaging techniques. Infection of A-427 non-small cell lung cancer cells with Ad-HAhSSTr2 or Ad-hSSTr2 resulted in similar expression of hSSTr2 by FACS analysis and binding assays using a (99m)Tc-labeled somatostatin analogue ((99m)Tc-P2045). HAhSSTr2 expression in A-427 cells was specific for infection with Ad-HAhSSTr2. FITC-labeled anti-HA antibody (FITC-HA) confirmed surface expression in live A-427 cells and the absence of internalization. Gamma camera imaging and gamma counter analysis of normal mice showed significantly greater (P<0.05) liver uptake of (99m)Tc-labeled anti-HA antibody ((99m)Tc-anti-HA) in mice injected i.v. 48 h earlier with Ad-HAhSSTr2 (53.6+/-6.9% ID/g) as compared to mice similarly injected with Ad-hSSTr2 (9.0+/-1.3% ID/g). In a mouse tumor model, imaging detected increased tumor localization of (99m)Tc-anti-HA due to direct intratumor injection Ad-HAhSSTr2. Gamma counter analysis confirmed significantly greater (P<0.05) uptake of (99m)Tc-anti-HA in tumors injected with Ad-HAhSSTr2 (12.5+/-4.1% ID/g) as compared to Ad-hSSTr2-infected tumors (5.1+/-1.5% ID/g). These studies demonstrate the feasibility of using an epitope-tagged reporter receptor for non-invasively imaging gene transfer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.