ArticleProceedings of the National Academy of Sciences of the United States of America2003
Drosophila DEG/ENaC pickpocket genes are expressed in the tracheal system, where they may be involved in liquid clearance.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 106 citations in OpenAlex.
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- The Drosophila DEG/ENaC PPK12 is a NaScientific reports · 2025Article
- ENaC is a host susceptibility factor to bacterial infections in cystic fibrosis context.Communications biology · 2025Article
- Airway specific deregulation of asthma-related serpins impairs tracheal architecture and oxygenation in D. melanogaster.Scientific reports · 2024Article
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- Bioelectric regulation of intestinal stem cells.Trends in cell biology · 2023Review
- Adult and Larval Tracheal Systems Exhibit Different Molecular Architectures inInternational journal of molecular sciences · 2023Article
- Functional analysis in a model sea anemone reveals phylogenetic complexity and a role in cnidocyte discharge of DEG/ENaC ion channels.Communications biology · 2023Article
- Insights into the genomic evolution of insects from cricket genomes.Communications biology · 2021Article
- A time course transcriptomic analysis of host and injected oncogenic cells reveals new aspects ofProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Salt Sensation and Regulation.Metabolites · 2021Review
- The roles of jim lovell and uninflatable in different endopolyploid larval tissues of Drosophila melanogaster.PloS one · 2020Article
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- Conserved function of the matriptase-prostasin proteolytic cascade during epithelial morphogenesis.PLoS genetics · 2019Article
- Physiological responses of the Drosophila labellum to amino acids.Journal of neurogenetics · 2018Article
- Endocrine regulation of airway clearance inProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Maggot Instructor: Semi-Automated Analysis of Learning and Memory inFrontiers in psychology · 2018Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The Drosophila tracheal system and mammalian airways are branching networks of tubular epithelia that deliver oxygen to the organism. In mammals, the epithelial Na(+) channel (ENaC) helps clear liquid from airways at the time of birth and removes liquid from the airspaces in adults. We tested the hypothesis that related Drosophila degenerin (DEG)/ENaC family members might play a similar role in the fly. Among 16 Drosophila DEG/ENaC genes, called pickpocket (PPK) genes, we found 9 expressed in the tracheal system. By in situ hybridization, expression appeared in late-stage embryos after tracheal tube formation, with individual PPK genes showing distinct temporal and spatial expression patterns as development progressed. Promoters for several PPK genes drove reporter gene expression in the larval and adult tracheal systems. Adding the DEG/ENaC channel blocker amiloride to the medium inhibited liquid clearance from the trachea of first instar larvae. Moreover, when RNA interference was used to silence PPK4 and PPK11, larvae failed to clear tracheal liquid. These data suggest substantial molecular diversity of DEG/ENaC channel expression in the Drosophila tracheal system where the PPK proteins likely play a role in Na(+) absorption. Extensive similarities between Drosophila and mammalian airways offer opportunities for genetic studies that may decipher further the structure and function of DEG/ENaC proteins and development of the airways.
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