ArticleApplied and environmental microbiology2003
Engineering of carbon distribution between glycolysis and sugar nucleotide biosynthesis in Lactococcus lactis.
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 57 citations in OpenAlex.
- Characterization of exopolysaccharide-producing lactic acid bacteria from Taiwanese ropy fermented milk and their application in low-fat fermented milk.Animal bioscience · 2022Article
- Microbes of traditional fermentation processes as synthetic biology chassis to tackle future food challenges.Frontiers in bioengineering and biotechnology · 2022Article
- Ebosin Attenuates the Inflammatory Responses Induced by TNF-Journal of immunology research · 2022Article
- Overexpression of Capsular Polysaccharide Biosynthesis Protein inJournal of microbiology and biotechnology · 2021Article
- Assessment of exopolysaccharide production byFood science & nutrition · 2020Article
- Systems Biology - A Guide for Understanding and Developing Improved Strains of Lactic Acid Bacteria.Frontiers in microbiology · 2019Review
- Effect of ebosin on modulating interleukin-1β-induced inflammatory responses in rat fibroblast-like synoviocytes.Cellular & molecular immunology · 2016Article
- Constructing a recombinant hyaluronic acid biosynthesis operon and producing food-grade hyaluronic acid in Lactococcus lactis.Journal of industrial microbiology & biotechnology · 2015Article
- Characterization of an Ebosin derivative produced by heterologous gene replacement in Streptomyces sp. 139.Microbial cell factories · 2014Article
- Recent advances in engineering the central carbon metabolism of industrially important bacteria.Microbial cell factories · 2012Review
- Effect of modified glucose catabolism on xanthan production in Xanthomonas oryzae pv. oryzae.Journal of industrial microbiology & biotechnology · 2012Article
- Physiological responses to folate overproduction in Lactobacillus plantarum WCFS1.Microbial cell factories · 2010Article
- Transcriptome analysis of the phytobacterium Xylella fastidiosa growing under xylem-based chemical conditions.Journal of biomedicine & biotechnology · 2010Article
- Reduced lipopolysaccharide phosphorylation in Escherichia coli lowers the elevated ori/ter ratio in seqA mutants.Molecular microbiology · 2009Article
- Metabolism associated with raised metabolic flux to sugar nucleotide precursors of exopolysaccharides in Lactobacillus delbrueckii subsp. bulgaricus.Journal of industrial microbiology & biotechnology · 2006Article
- Roles of thioredoxin reductase during the aerobic life of Lactococcus lactis.Journal of bacteriology · 2005Article
- Increased exopolysaccharide production in Lactococcus lactis due to increased levels of expression of the NIZO B40 eps gene cluster.Applied and environmental microbiology · 2003Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe the effects of modulating the activities of glucokinase, phosphofructokinase, and phosphoglucomutase on the branching point between sugar degradation and the biosynthesis of sugar nucleotides involved in the production of exopolysaccharide biosynthesis by Lactococcus lactis. This was realized by using a described isogenic L. lactis mutant with reduced enzyme activities or by controlled expression of the well-characterized genes for phosphoglucomutase or glucokinase from Escherichia coli or Bacillus subtilis, respectively. The role of decreased metabolic flux was studied in L. lactis strains with decreased phosphofructokinase activities. The concomitant reduction of the activities of phosphofructokinase and other enzymes encoded by the las operon (lactate dehydrogenase and pyruvate kinase) resulted in significant changes in the concentrations of sugar-phosphates. In contrast, a >25-fold overproduction of glucokinase resulted in 7-fold-increased fructose-6-phosphate levels and 2-fold-reduced glucose-1-phosphate and glucose-6-phosphate levels. However, these increased sugar-phosphate concentrations did not affect the levels of sugar nucleotides. Finally, an approximately 100-fold overproduction of phosphoglucomutase resulted in 5-fold-increased levels of both UDP-glucose and UDP-galactose. While the increased concentrations of sugar-phosphates or sugar nucleotides did not significantly affect the production of exopolysaccharides, they demonstrate the metabolic flexibility of L. lactis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.