ArticleApplied and environmental microbiology2003
Small, acid-soluble proteins as biomarkers in mass spectrometry analysis of Bacillus spores.
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed.
- TrackingACS omega · 2026Review
- Integrated genomics and proteomics analysis of Paenibacillus peoriae IBSD35 and insights into its antimicrobial characteristics.Scientific reports · 2022Article
- Raman spectroscopy reveals alteration of spore compositions under different nutritional conditions in Lysinibacillus boronitolerans YS11.Journal of microbiology (Seoul, Korea) · 2021Article
- Microwave supported hydrolysis preparesInternational journal of mass spectrometry · 2019Article
- Article
- Bacillus Classification Based on Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry-Effects of Culture Conditions.Scientific reports · 2017Article
- Identification of Highly Pathogenic Microorganisms by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry: Results of an Interlaboratory Ring Trial.Journal of clinical microbiology · 2015Article
- Identification and validation of specific markers of Bacillus anthracis spores by proteomics and genomics approaches.Molecular & cellular proteomics : MCP · 2014Article
- Rapid identification of Bacillus anthracis spores in suspicious powder samples by using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS).Applied and environmental microbiology · 2013Article
- Identifying experimental surrogates for Bacillus anthracis spores: a review.Investigative genetics · 2010Article
- Targeted proteomics approach to species-level identification of Bacillus thuringiensis spores by AP-MALDI-MS.Journal of the American Society for Mass Spectrometry · 2010Article
- Identification of Bacillus anthracis by using matrix-assisted laser desorption ionization-time of flight mass spectrometry and artificial neural networks.Applied and environmental microbiology · 2009Article
- Parameters contributing to efficient ion generation in aerosol MALDI mass spectrometry.Journal of the American Society for Mass Spectrometry · 2008Article
- MALDI-TOF-MS analysis of bacterial spores: wet heat-treatment as a new releasing technique for biomarkers and the influence of different experimental parameters and microbiological handling.Journal of the American Society for Mass Spectrometry · 2004Article
- Species differentiation of a diverse suite of Bacillus spores by mass spectrometry-based protein profiling.Applied and environmental microbiology · 2004Article
- Advances in mass spectrometry for the identification of pathogens.Mass spectrometry reviewsReview
Corrections and comments
- Erratum issued
Authors and funding
5 authors.
Funding
Abstract
The use of 1 N HCl for extraction of small, acid-soluble proteins (SASP) from different Bacillus spore species was examined. The extracts were analyzed by high-performance liquid chromatography and matrix-assisted laser desorption mass spectrometry and were found to be both qualitatively and quantitatively superior to extraction by acetonitrile-5% trifluoroacetic acid (70:30, vol/vol). Both major and minor alpha/beta- and gamma-type SASP were characterized by their molecular masses or tryptic peptide maps and by searches of both protein and unannotated genome databases. For all but 1 pair (B. cereus T and B. thuringiensis subsp. Kurstaki) among the 11 variants studied the suites of SASP masses are distinctive, consistent with the use of these proteins as potential biomarkers for spore identification by mass spectrometry.
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