ArticleApplied and environmental microbiology2003
Growth of a Dehalococcoides-like microorganism on vinyl chloride and cis-dichloroethene as electron acceptors as determined by competitive PCR.
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 52 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
52 citing papers in PubMed, 261 citations in OpenAlex.
- Sorption-mediated organohalide respiration biokinetics of tetrachloroethene.Water research · 2026Article
- Reductive degradation of carbon tetrachloride using tree leaf polyphenol-iron complexes for groundwater remediation.RSC advances · 2025Article
- Dehalococcoides mccartyi strain NIT01 grows more stably in vessels made of pure titanium rather than the stainless alloy SUS304.Environmental microbiology reports · 2023Article
- Review
- Dehalogenation of Chlorinated Ethenes to Ethene by a Novel Isolate, "Applied and environmental microbiology · 2022Article
- Acetylene-Fueled Trichloroethene Reductive Dechlorination in a Groundwater Enrichment Culture.mBio · 2021Article
- Effects of Arsenic on Trichloroethene-Dechlorination Activities ofEnvironmental science & technology · 2020Article
- Structural dynamics and transcriptomic analysis of Dehalococcoides mccartyi within a TCE-Dechlorinating community in a completely mixed flow reactor.Water research · 2019Article
- Detection of Organohalide-Respiring Enzyme Biomarkers at a Bioaugmented TCE-Contaminated Field Site.Frontiers in microbiology · 2019Article
- Chlorinated Electron Acceptor Abundance Drives Selection ofFrontiers in microbiology · 2018Article
- Secondary compound hypothesis revisited: Selected plant secondary metabolites promote bacterial degradation of cis-1,2-dichloroethylene (cDCE).Scientific reports · 2017Article
- Isolation and genomic characterization of a Dehalococcoides strain suggests genomic rearrangement during culture.Scientific reports · 2017Article
- Microbial degradation of chloroethenes: a review.Environmental science and pollution research international · 2017Review
- Effects of Sulfate Reduction on Trichloroethene Dechlorination by Dehalococcoides-Containing Microbial Communities.Applied and environmental microbiology · 2017Article
- Metagenomic and Metatranscriptomic Analyses Reveal the Structure and Dynamics of a Dechlorinating Community Containing Dehalococcoides mccartyi and Corrinoid-Providing Microorganisms under Cobalamin-Limited Conditions.Applied and environmental microbiology · 2017Article
- Nocardioides, Sediminibacterium, Aquabacterium, Variovorax, and Pseudomonas linked to carbon uptake during aerobic vinyl chloride biodegradation.Environmental science and pollution research international · 2016Article
- Combination of aquifer thermal energy storage and enhanced bioremediation: resilience of reductive dechlorination to redox changes.Applied microbiology and biotechnology · 2016Article
- Loop-Mediated Isothermal Amplification (LAMP) for Rapid Detection and Quantification of Dehalococcoides Biomarker Genes in Commercial Reductive Dechlorinating Cultures KB-1 and SDC-9.Applied and environmental microbiology · 2016Article
- The roles of methanogens and acetogens in dechlorination of trichloroethene using different electron donors.Environmental science and pollution research international · 2015Article
- Biodegradation of high concentrations of halomethanes by a fermentative enrichment culture.AMB Express · 2014Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A competitive PCR (cPCR) assay targeting 16S ribosomal DNA was developed to enumerate growth of a Dehalococcoides-like microorganism, bacterium VS, from a mixed culture catalyzing the reductive dehalogenation of cis-1,2-dichloroethene (cDCE) and vinyl chloride (VC), with hydrogen being used as an electron donor. The growth of bacterium VS was found to be coupled to the dehalogenation of VC and cDCE, suggesting unique metabolic capabilities. The average growth yield was (5.2 +/- 1.5) x 10(8) copies of the 16S rRNA gene/ micromol of Cl(-) (number of samples, 10), with VC being used as the electron acceptor and hydrogen as the electron donor. The maximum VC utilization rate (q) was determined to be 7.8 x 10(-10) micromol of Cl(-) (copy(-1) day(-1)), indicating a maximum growth rate of 0.4 day(-1). These average growth yield and q values agree well with values found previously for dechlorinating cultures. Decay coefficients were determined with growth (0.05 day(-1)) and no-growth (0.09 day(-1)) conditions. An important limitation of this cPCR assay was its inability to discriminate between active and inactive cells. This is an essential consideration for kinetic studies.
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