ArticleApplied and environmental microbiology2003
Heat and osmotic stress responses of probiotic Lactobacillus rhamnosus HN001 (DR20) in relation to viability after drying.
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 220 citations in OpenAlex.
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- Probiotic Development Strategy Centered on Stability and Regulatory Considerations.Comprehensive reviews in food science and food safety · 2026Review
- Whole-genome analysis ofMicrobiology spectrum · 2025Article
- Lacticaseibacillus paracasei AD22 Stress Response in Brined White Cheese Matrix: In Vitro Probiotic Profiles and Molecular Characterization.Probiotics and antimicrobial proteins · 2025Article
- A stress-tolerant strain Rhodococcus sp. WH103 was isolated and co-immobilized to more efficiently degrade phenazine-1-carboxylic acid.Scientific reports · 2025Article
- Comprehensive Phenotypic Characterization and Genomic Analysis Unveil the Probiotic Potential ofAnimals : an open access journal from MDPI · 2025Article
- A Closer Look at the Potential Mechanisms of Action of Protective Agents Used in the Drying of Microorganisms: A Review.Microorganisms · 2025Review
- Genomic insights of Lactiplantibacillus plantarum CNPC024: a potential probiotic strain producing immune-boosting tryptophan-derived metabolites.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Key factors for the survival of Lactiplantibacillus plantarum IDCC 3501 in manufacturing and storage.Applied microbiology and biotechnology · 2024Article
- Probiotics and Paraprobiotics: Effects on Microbiota-Gut-Brain Axis and Their Consequent Potential in Neuropsychiatric Therapy.Probiotics and antimicrobial proteins · 2024Review
- Fermentation Characteristics, Antinutritional Factor Level and Flavor Compounds of Soybean Whey Yogurt.Foods (Basel, Switzerland) · 2024Article
- Genomic and Functional Evaluation of TwoGenes · 2024Article
- Enhanced Cell Wall and Cell Membrane Activity Promotes Heat Adaptation ofInternational journal of molecular sciences · 2023Article
- Transcriptome-phenotype matching analysis of how nitrogen sources influence Lacticaseibacillus rhamnosus tolerance to heat stress and oxidative stress.Microbial cell factories · 2022Article
- The Effect of Proline on the Freeze-Drying Survival Rate ofInternational journal of molecular sciences · 2022Article
- Influence of nitrogen sources on the tolerance of Lacticaseibacillus rhamnosus to heat stress and oxidative stress.Journal of industrial microbiology & biotechnology · 2022Article
- Article
- Phenolic compound profile of probiotic (Lacticaseibacillus rhamnosus LR5) fortified vegetable tablet and probiotic survival in the simulated gastrointestinal tract.Scientific reports · 2022Article
- Probiotic growth in skin-like conditions.AIMS microbiology · 2022Article
- Physiological and transcriptomic analyses revealed the change of main flavor substance ofFrontiers in nutrition · 2022Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The viability of lactic acid bacteria in frozen, freeze-dried, and air-dried forms is of significant commercial interest to both the dairy and food industries. In this study we observed that when prestressed with either heat (50 degrees C) or salt (0.6 M NaCl), Lactobacillus rhamnosus HN001 (also known as DR20) showed significant (P < 0.05) improvement in viability compared with the nonstressed control culture after storage at 30 degrees C in the dried form. To investigate the mechanisms underlying this stress-related viability improvement in L. rhamnosus HN001, we analyzed protein synthesis in cultures subjected to different growth stages and stress conditions, using two-dimensional gel electrophoresis and N-terminal sequencing. Several proteins were up- or down-regulated after either heat or osmotic shock treatments. Eleven proteins were positively identified, including the classical heat shock proteins GroEL and DnaK and the glycolytic enzymes glyceraldehyde-3-phosphate dehydrogenase, lactate dehydrogenase, enolase, phosphoglycerate kinase, and triose phosphate isomerase, as well as tagatose 1,6-diphosphate aldolase of the tagatose pathway. The phosphocarrier protein HPr (histidine-containing proteins) was up-regulated in cultures after the log phase irrespective of the stress treatments used. The relative synthesis of an ABC transport-related protein was also up-regulated after shock treatments. Carbohydrate analysis of cytoplasmic contents showed higher levels (20 +/- 3 microg/mg of protein) in cell extracts (CFEs) derived from osmotically stressed cells than in the unstressed control (15 +/- 3 microg/mg of protein). Liquid chromatography of these crude carbohydrate extracts showed significantly different profiles. Electrospray mass spectrometry analysis of CFEs revealed, in addition to normal mono-, di-, tri-, and tetrasaccharides, the presence of saccharides modified with glycerol.
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