ArticleApplied and environmental microbiology2003
Characterization of chitinase genes from an alkaliphilic actinomycete, Nocardiopsis prasina OPC-131.
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 77 citations in OpenAlex.
- The howler monkey genome provides new insights into the distinctive howling and folivorous adaptations of New World monkeys.Genome biology · 2026Article
- Domain gain or loss in a fungal chitinase enables specialization towards antagonism or immune suppression.Nature communications · 2026Article
- Microbial chitinases in the plant holobiont: ecological roles and structural determinants.FEMS microbiology reviews · 2026Review
- Article
- Microbial chitinases and their relevance in various industries.Folia microbiologica · 2023Review
- The Diversity, Metabolomics Profiling, and the Pharmacological Potential of Actinomycetes Isolated from the Estremadura Spur Pockmarks (Portugal).Marine drugs · 2021Article
- Molecular Characterization of Four Alkaline Chitinases from Three Chitinolytic Bacteria Isolated from a Mudflat.International journal of molecular sciences · 2021Article
- Microbial decomposition of crustacean shell for production of bioactive metabolites and study of its fertilizing potential.Environmental science and pollution research international · 2021Article
- Biocontrol potential of chitinases produced by newly isolated Chitinophaga sp. S167.World journal of microbiology & biotechnology · 2020Article
- Cloning and characterization of a chitinase fromBiotechnology reports (Amsterdam, Netherlands) · 2018Article
- Transcriptomic and Ectoine Analysis of HalotolerantFrontiers in microbiology · 2018Article
- Sorghum husk biomass as a potential substrate for production of cellulolytic and xylanolytic enzymes by Nocardiopsis sp. KNU.3 Biotech · 2017Article
- Chitinase-producing bacteria and their role in biocontrol.AIMS microbiology · 2017Review
- Review
- An Alternative Bacterial Expression System UsingIranian journal of biotechnology · 2015Article
- Thermophilic and alkaliphilic Actinobacteria: biology and potential applications.Frontiers in microbiology · 2015Review
- Exploring the functional soil-microbe interface and exoenzymes through soil metaexoproteomics.The ISME journal · 2014Article
- Isolation, Characterization and Selection of Avermectin-Producing Streptomyces avermitilis Strains From Soil Samples.Jundishapur journal of microbiology · 2014Article
- Bacterial chitin degradation-mechanisms and ecophysiological strategies.Frontiers in microbiology · 2013Article
- Bacterial chitin utilisation at extremely haloalkaline conditions.Extremophiles : life under extreme conditions · 2012Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An alkaliphilic actinomycete, Nocardiopsis prasina OPC-131, secretes chitinases, ChiA, ChiB, and ChiB Delta, in the presence of chitin. The genes encoding ChiA and ChiB were cloned and sequenced. The open reading frame (ORF) of chiA encoded a protein of 336 amino acids with a calculated molecular mass of 35,257 Da. ChiA consisted of only a catalytic domain and showed a significant homology with family 18 chitinases. The chiB ORF encoded a protein of 296 amino acids with a calculated molecular mass of 31,500 Da. ChiB is a modular enzyme consisting of a chitin-binding domain type 3 (ChtBD type 3) and a catalytic domain. The catalytic domain of ChiB showed significant similarity to Streptomyces family 19 chitinases. ChiB Delta was the truncated form of ChiB lacking ChtBD type 3. Expression plasmids coding for ChiA, ChiB, and ChiB Delta were constructed to investigate the biochemical properties of these recombinant proteins. These enzymes showed pHs and temperature optima similar to those of native enzymes. ChiB showed more efficient hydrolysis of chitin and stronger antifungal activity than ChiB Delta, indicating that the ChtBD type 3 of ChiB plays an important role in the efficient hydrolysis of chitin and in antifungal activity. Furthermore, the finding of family 19 chitinase in N. prasina OPC-131 suggests that family 19 chitinases are distributed widely in actinomycetes other than the genus Streptomyces.
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