ArticleApplied and environmental microbiology2003
Identification of a high-affinity phosphate transporter gene in a prasinophyte alga, Tetraselmis chui, and its expression under nutrient limitation.
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 81 citations in OpenAlex.
- Dealing with phosphorus deficiency: contrasting strategies in marine phytoplankton and bacteria.ISME communications · 2026Article
- A novel algae-assisted sequencing batch and intermittent air-lift bioreactor (A-SBIAB) using polyester filament-based carriers for piggery wastewater treatment.Bioprocess and biosystems engineering · 2025Article
- Giant Virus Infection Signatures Are Modulated by Euphotic Zone Depth Strata and Iron Regimes of the Subantarctic Southern Ocean.mSystems · 2023Article
- Physiological responses, tolerance, and remediation strategies in plants exposed to metalloids.Environmental science and pollution research international · 2021Review
- Selection and validation of reference genes for quantitative real-time PCR in the green microalgae Tetraselmis chui.PloS one · 2021Article
- Surviving Starvation: Proteomic and Lipidomic Profiling of Nutrient Deprivation in the Smallest Known Free-Living Eukaryote.Metabolites · 2020Article
- Article
- Conserved Transcriptional Responses to Nutrient Stress in Bloom-Forming Algae.Frontiers in microbiology · 2017Article
- Article
- Changes in gene expression of Prymnesium parvum induced by nitrogen and phosphorus limitation.Frontiers in microbiology · 2015Article
- De novo assembly of Aureococcus anophagefferens transcriptomes reveals diverse responses to the low nutrient and low light conditions present during blooms.Frontiers in microbiology · 2014Article
- Responses of phosphate transporter gene and alkaline phosphatase in Thalassiosira pseudonana to phosphine.PloS one · 2013Article
- Characterization of the proteomic profiles of the brown tide alga Aureoumbra lagunensis under phosphate- and nitrogen-limiting conditions and of its phosphate limitation-specific protein with alkaline phosphatase activity.Applied and environmental microbiology · 2012Article
- Phosphate transporters in marine phytoplankton and their viruses: cross-domain commonalities in viral-host gene exchanges.Environmental microbiology · 2012Article
- Transcriptomic response of the red tide dinoflagellate, Karenia brevis, to nitrogen and phosphorus depletion and addition.BMC genomics · 2011Article
- Genome sequence of Ostreococcus tauri virus OtV-2 throws light on the role of picoeukaryote niche separation in the ocean.Journal of virology · 2011Article
- Nutrient-regulated transcriptional responses in the brown tide-forming alga Aureococcus anophagefferens.Environmental microbiology · 2011Article
- Identification and Characterization of Cell Wall Proteins of a Toxic Dinoflagellate Alexandrium catenella Using 2-D DIGE and MALDI TOF-TOF Mass Spectrometry.Evidence-based complementary and alternative medicine : eCAM · 2011Article
- Proteome changes driven by phosphorus deficiency and recovery in the brown tide-forming alga Aureococcus anophagefferens.PloS one · 2011Article
- Article
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A high-affinity phosphate transporter gene, TcPHO, was isolated from a growth-dependent subtracted cDNA library of the marine unicellular alga Tetraselmis chui. The full-length cDNA of TcPHO obtained by 5' and 3' rapid amplification of cDNA ends was 1,993 bp long and encoded an open reading frame consisting of 610 amino acids. The deduced amino acid sequence of TcPHO exhibited 51.6 and 49.8% similarity to the amino acid sequences of PHO89 from Saccharomyces cerevisiae and PHO4 from Neurospora crassa, respectively. In addition, hydrophobicity and secondary structure analyses revealed 12 conserved transmembrane domains that were the same as those found in PHO89 and PHO4. The expression of TcPHO mRNA was dependent on phosphate availability. With a low-phosphate treatment, the TcPHO mRNA concentration increased sharply to 2.72 fmol micro g of total RNA(-1) from day 1 to day 2 and remained at this high level from days 2 to 4. Furthermore, rescue treatment with either phosphate or p-nitrophenyl phosphate effectively inhibited TcPHO mRNA expression. In contrast, TcPHO mRNA expression stayed at a low level (range, 0.25 to 0.28 fmol micro g of total RNA(-1)) under low-nitrate conditions. The expression pattern suggests that TcPHO can be used as a molecular probe for monitoring phosphorus stress in T. chui.
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