ArticleApplied and environmental microbiology2003
Isolation and molecular characterization of pMG160, a mobilizable cryptic plasmid from Rhodobacter blasticus.
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 30 citations in OpenAlex.
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- Tools for genetic engineering and gene expression control inApplied and environmental microbiology · 2024Article
- Tools for Genetic Engineering and Gene Expression Control inbioRxiv : the preprint server for biology · 2023Article
- Construction of a Rhodobacter sphaeroides Strain That Efficiently Produces Hydrogen Gas from Acetate without Poly(β-Hydroxybutyrate) Accumulation: Insight into the Role of PhaR in Acetate Metabolism.Applied and environmental microbiology · 2022Article
- The transition of Rhodobacter sphaeroides into a microbial cell factory.Biotechnology and bioengineering · 2021Review
- Introduction of Glyoxylate Bypass Increases Hydrogen Gas Yield from Acetate and l-Glutamate inApplied and environmental microbiology · 2019Article
- Use of new strains of Rhodobacter sphaeroides and a modified simple culture medium to increase yield and facilitate purification of the reaction centre.Photosynthesis research · 2014Article
- Plasmids of carotenoid-producing Paracoccus spp. (Alphaproteobacteria) - structure, diversity and evolution.PloS one · 2013Article
- A novel component of the Rhodobacter sphaeroides Fla1 flagellum is essential for motor rotation.Journal of bacteriology · 2012Article
- Coproporphyrin excretion and low thiol levels caused by point mutation in the Rhodobacter sphaeroides S-adenosylmethionine synthetase gene.Journal of bacteriology · 2010Article
- Inducible-expression plasmid for Rhodobacter sphaeroides and Paracoccus denitrificans.Applied and environmental microbiology · 2009Article
- DNA signatures for detecting genetic engineering in bacteria.Genome biology · 2008Article
- Nucleotide sequence and evolution of the five-plasmid complement of the phytopathogen Pseudomonas syringae pv. maculicola ES4326.Journal of bacteriology · 2004Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A 3.4-kb cryptic plasmid was obtained from a new isolate of Rhodobacter blasticus. This plasmid, designated pMG160, was mobilizable by the conjugative strain Escherichia coli S17.1 into Rhodobacter sphaeroides, Rhodobacter capsulatus, and Rhodopseudomonas palustris. It replicated in the latter strains but not in Rhodospirillum rubrum, Rhodocyclus gelatinosus, or Bradyrhizobium species. Plasmid pMG160 was stably maintained in R. sphaeroides for more than 100 generations in the absence of selection but showed segregational instability in R. palustris. Instability in R. palustris correlated with a decrease in plasmid copy number compared to the copy number in R. sphaeroides. The complete nucleotide sequence of plasmid pMG160 contained three open reading frames (ORFs). The deduced amino acid sequences encoded by ORF1 and ORF2 showed high degrees of homology to the MobS and MobL proteins that are involved in plasmid mobilization of certain plasmids. Based on homology with the Rep protein of several other plasmids, ORF3 encodes a putative rep gene initiator of plasmid replication. The functions of these sequences were demonstrated by deletion mapping, frameshift analysis, and analysis of point mutations. Two 6.1-kb pMG160-based E. coli-R. sphaeroides shuttle cloning vectors were constructed and designated pMG170 and pMG171. These two novel shuttle vectors were segregationally stable in R. sphaeroides growing under nonselective conditions.
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