ArticleThe Plant cell2003
Plant gamma-tubulin interacts with alphabeta-tubulin dimers and forms membrane-associated complexes.
Article in The Plant cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 116 citations in OpenAlex.
- Pan-Genome Analysis of theGenes · 2026Article
- Genome-wide identification and evolution of the tubulin gene family in Camelina sativa.BMC genomics · 2024Article
- γ-Tubulin in microtubule nucleation and beyond.Frontiers in cell and developmental biology · 2022Review
- The parent-of-origin lncRNA MISSEN regulates rice endosperm development.Nature communications · 2021Article
- γ-Tubulin Complexes and Fibrillar Arrays: Two Conserved High Molecular Forms with Many Cellular Functions.Cells · 2021Review
- Genome-Wide Analysis of Tubulin Gene Family in Cassava and Expression of Family MemberPlants (Basel, Switzerland) · 2021Article
- Review
- Regulation of microtubule nucleation mediated by γ-tubulin complexes.Protoplasma · 2017Review
- Review
- Abiotic stress responses in plant roots: a proteomics perspective.Frontiers in plant science · 2014Review
- Overexpressed TPX2 causes ectopic formation of microtubular arrays in the nuclei of acentrosomal plant cells.Journal of experimental botany · 2013Article
- Fodrin in centrosomes: implication of a role of fodrin in the transport of gamma-tubulin complex in brain.PloS one · 2013Article
- Interactions of an Arabidopsis RanBPM homologue with LisH-CTLH domain proteins revealed high conservation of CTLH complexes in eukaryotes.BMC plant biology · 2012Article
- The Arabidopsis ARCP protein, CSI1, which is required for microtubule stability, is necessary for root and anther development.The Plant cell · 2012Article
- A bacterial acetyltransferase destroys plant microtubule networks and blocks secretion.PLoS pathogens · 2012Article
- Cytoskeleton in mast cell signaling.Frontiers in immunology · 2012Article
- Feeding cells induced by phytoparasitic nematodes require γ-tubulin ring complex for microtubule reorganization.PLoS pathogens · 2011Article
- Article
- Tyrosine phosphorylation of plant tubulin.Planta · 2008Article
- Recovery of microtubules on the blepharoplast of Ceratopteris spermatogenous cells after oryzalin treatment.Protoplasma · 2008Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
gamma-Tubulin is assumed to participate in microtubule nucleation in acentrosomal plant cells, but the underlying molecular mechanisms are still unknown. Here, we show that gamma-tubulin is present in protein complexes of various sizes and different subcellular locations in Arabidopsis and fava bean. Immunoprecipitation experiments revealed an association of gamma-tubulin with alphabeta-tubulin dimers. gamma-Tubulin cosedimented with microtubules polymerized in vitro and localized along their whole length. Large gamma-tubulin complexes resistant to salt treatment were found to be associated with a high-speed microsomal fraction. Blue native electrophoresis of detergent-solubilized microsomes showed that the molecular mass of the complexes was >1 MD. Large gamma-tubulin complexes were active in microtubule nucleation, but nucleation activity was not observed for the smaller complexes. Punctate gamma-tubulin staining was associated with microtubule arrays, accumulated with short kinetochore microtubules interacting in polar regions with membranes, and localized in the vicinity of nuclei and in the area of cell plate formation. Our results indicate that the association of gamma-tubulin complexes with dynamic membranes might ensure the flexibility of noncentrosomal microtubule nucleation. Moreover, the presence of other molecular forms of gamma-tubulin suggests additional roles for this protein species in microtubule organization.
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