ArticleThe Journal of physiology2003
Somatic exocytosis of serotonin mediated by L-type calcium channels in cultured leech neurones.
Article in The Journal of physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.
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Who cites it
36 citing papers in PubMed, 1 synthesis or guideline pooled it, 64 citations in OpenAlex.
- Retinal VIP-amacrine cells: their development, structure, and function.Eye (London, England) · 2024Pooled it
- Differential Autoregulation of Serotonin Secretion at Different Structures of Serotonergic Neurons.International journal of molecular sciences · 2026Review
- The Thermodynamically Expensive Contribution of Three Calcium Sources to Somatic Release of Serotonin.International journal of molecular sciences · 2022Review
- Article
- Activation of the glucocorticoid receptor rapidly triggers calcium-dependent serotonin release in vitro.CNS neuroscience & therapeutics · 2021Article
- Extrasynaptic Communication.Frontiers in molecular neuroscience · 2021Article
- Electrochemistry at the Synapse.Annual review of analytical chemistry (Palo Alto, Calif.) · 2019Review
- Serotonin induces or inhibits neuritic regeneration of leech CNS neurons depending on neuronal identity.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2019Article
- Thermodynamic Efficiency of Somatic Exocytosis of Serotonin.Frontiers in physiology · 2019Article
- Label-free imaging of neurotransmitters in live brain tissue by multi-photon ultraviolet microscopy.Neuronal signaling · 2018Review
- Somato-Dendritic Regulation of Raphe Serotonin Neurons; A Key to Antidepressant Action.Frontiers in neuroscience · 2018Review
- Article
- Extrasynaptic Neurotransmission Mediated by Exocytosis and Diffusive Release of Transmitter Substances.Frontiers in synaptic neuroscience · 2018Review
- On the Basis of Synaptic Integration Constancy during Growth of a Neuronal Circuit.Frontiers in cellular neuroscience · 2016Article
- Effect of amyloids on the vesicular machinery: implications for somatic neurotransmission.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Review
- Serotonin release from the neuronal cell body and its long-lasting effects on the nervous system.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Article
- Label-free dopamine imaging in live rat brain slices.ACS chemical neuroscience · 2014Article
- The self-regulation of neurotransmitter release.Frontiers in cellular neuroscience · 2014Article
- Intracellular calcium stores drive slow non-ribbon vesicle release from rod photoreceptors.Frontiers in cellular neuroscience · 2014Article
- Exocytosis of serotonin from the neuronal soma is sustained by a serotonin and calcium-dependent feedback loop.Frontiers in cellular neuroscience · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We studied somatic exocytosis of serotonin and its mediation by L-type calcium (Ca2+) channels in cultured Retzius neurones of the leech. Exocytosis was induced by trains of impulses at different frequencies or by depolarisation with 40 mM potassium (K+), and was quantified by use of the fluorescent dye FM 1-43. Stimulation increased the membrane fluorescence and produced a pattern of FM 1-43 fluorescent spots of 1.28 +/- 0.01 microm in diameter, provided that Ca2+ was present in the bathing fluid. Individual spots lost their stain during depolarisation with 40 mM K+. Electron micrographs showed clusters of dense core vesicles, some of which were in contact with the cell membrane. Presynaptic structures with clear vesicles were absent from the soma. The number of fluorescent spots per soma, but not their diameter or their fluorescence intensity, depended on the frequency of stimulation. Trains at 1 Hz produced 19.5 +/- 5 spots per soma, 77.9 +/- 13.9 spots per soma were produced at 10 Hz and 91.5 +/- 16.9 spots per soma at 20 Hz. Staining patterns were similar for neurones in culture and in situ. In the presence of the L-type Ca2+ channel blocker nimodipine (10 microM), a 20 Hz train produced only 22.9 +/- 6.4 spots per soma, representing a 75 % reduction compared to control cells (P < 0.05). Subsequent incubation with 10 mM caffeine to induce Ca2+ release from intracellular stores increased the number of spots to 73.22 +/- 12.5. Blockers of N-, P-, Q- or invertebrate Ca2+ channels did not affect somatic exocytosis. Our results suggest that somatic exocytosis by neurones shares common mechanisms with excitable endocrine cells.
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