ArticleThe Journal of physiology2003
Multiple conductance states of single Ca2+-activated Cl- channels in rabbit pulmonary artery smooth muscle cells.
Article in The Journal of physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 48 citations in OpenAlex.
- Phosphatidylinositol 4,5-Bisphosphate and Cholesterol Regulators of the Calcium-Activated Chloride Channels TMEM16A and TMEM16B.Advances in experimental medicine and biology · 2023Article
- Molecular mechanisms of activation and regulation of ANO1-Encoded CaChannels (Austin, Tex.) · 2021Review
- Ouabain-Induced Cell Death and Survival. Role of α1-Na,K-ATPase-Mediated Signaling and [NaFrontiers in physiology · 2020Review
- TMEM16A is implicated in the regulation of coronary flow and is altered in hypertension.British journal of pharmacology · 2019Article
- Properties of single-channel and whole cell ClAmerican journal of physiology. Cell physiology · 2019Article
- Influence of intracellular Ca2+ and alternative splicing on the pharmacological profile of ANO1 channels.American journal of physiology. Cell physiology · 2016Article
- The molecular basis of the genesis of basal tone in internal anal sphincter.Nature communications · 2016Article
- Mechanisms underlying spontaneous constrictions of postcapillary venules in the rat stomach.Pflugers Archiv : European journal of physiology · 2016Article
- Molecular and functional significance of Ca(2+)-activated Cl(-) channels in pulmonary arterial smooth muscle.Pulmonary circulation · 2015Review
- Role of volume-regulated and calcium-activated anion channels in cell volume homeostasis, cancer and drug resistance.Channels (Austin, Tex.) · 2015Review
- Inhibitory role of phosphatidylinositol 4,5-bisphosphate on TMEM16A-encoded calcium-activated chloride channels in rat pulmonary artery.British journal of pharmacology · 2014Article
- Increase in cytosolic Ca2+ produced by hypoxia and other depolarizing stimuli activates a non-selective cation channel in chemoreceptor cells of rat carotid body.The Journal of physiology · 2014Article
- Pharmacological characterization of TMEM16A currents.Channels (Austin, Tex.) · 2014Article
- Anoctamin 1 mediates thermal pain as a heat sensor.Current neuropharmacology · 2013Article
- Potent vasorelaxant activity of the TMEM16A inhibitor T16A(inh) -A01.British journal of pharmacology · 2013Article
- Hypoxic pulmonary vasoconstriction.Physiological reviews · 2012Review
- International Union of Basic and Clinical Pharmacology. LXXXV: calcium-activated chloride channels.Pharmacological reviews · 2012Review
- TMEM16A channels generate Ca²⁺-activated Cl⁻ currents in cerebral artery smooth muscle cells.American journal of physiology. Heart and circulatory physiology · 2011Article
- Expression profile and protein translation of TMEM16A in murine smooth muscle.American journal of physiology. Cell physiology · 2010Article
- Anoctamin/TMEM16 family members are Ca2+-activated Cl- channels.The Journal of physiology · 2009Review
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Ca2+-activated Cl- channels contribute to agonist-evoked contraction and spontaneous activity in some smooth muscle preparations. Patch pipette techniques were used to study the properties of single Ca2+-activated Cl- channels in freshly dispersed rabbit pulmonary artery myocytes. In the cell-attached recording mode, two conductance states of 3.5 and 1.8 pS were recorded either spontaneously or in response to increasing [Ca2+]i. With inside-out patches, the 3.5 pS channel current predominated at 50 nM [Ca2+]i, but at 500 nM [Ca2+]i most channels opened to the 1.8 pS level and an additional 1.2 pS channel conductance was resolved. At 1 microM [Ca2+]i all of the Cl- channels opened either to the 1.8 pS or 1.2 pS level. In 0 [Ca2+]i, no channel activity was observed at -100 mV to +100 mV, but with 10-250 nM [Ca2+]i the total single channel open probability (NP(o)) increased with depolarisation. This voltage dependence was not seen at higher values of [Ca2+]i. The plot of NPo vs. [Ca2+]i yielded Ca2+ affinity constants of 8 and 250 nM and Hill slopes of 1.3 and 2.3 at +100 and -100 mV, respectively. The distribution of open times was fitted by two exponentials of about 5 and 30 ms, which were neither voltage nor Ca2+ dependent. Replacement of external Cl- by I- shifted the reversal potential by about -30 mV and lengthened the longer of the two mean open times without significant effects on other kinetic parameters. Based on these data, a model for the activation of Ca2+-activated Cl- channels is proposed.
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