ArticleJournal of bacteriology2003
Analysis of DNA binding and transcriptional activation by the LysR-type transcriptional regulator CbbR of Xanthobacter flavus.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 54 citations in OpenAlex.
- Fundamentals and Exceptions of the LysR-type Transcriptional Regulators.ACS synthetic biology · 2024Review
- Inactivation of poly(3-hydroxybutyrate) (PHB) biosynthesis in 'Knallgas' bacterium Xanthobacter sp. SoF1.AMB Express · 2023Article
- The LysR-Type Transcriptional Regulator BsrA (PA2121) Controls Vital Metabolic Pathways inmSystems · 2021Article
- A LysR Family Transcriptional Regulator Modulates Burkholderia cenocepacia Biofilm Formation and Protease Production.Applied and environmental microbiology · 2021Article
- A novel 3-hydroxypropionic acid-inducible promoter regulated by the LysR-type transcriptional activator protein MmsR of Pseudomonas denitrificans.Scientific reports · 2019Article
- Unraveling RubisCO Form I and Form II Regulation in an Uncultured Organism from a Deep-Sea Hydrothermal Vent via Metagenomic and Mutagenesis Studies.Frontiers in microbiology · 2017Article
- PnpM, a LysR-Type Transcriptional Regulator Activates the Hydroquinone Pathway inFrontiers in microbiology · 2017Article
- Proteomic analysis of organic sulfur compound utilisation in Advenella mimigardefordensis strain DPN7T.PloS one · 2017Article
- OxyR-dependent formation of DNA methylation patterns in OpvABOFF and OpvABON cell lineages of Salmonella enterica.Nucleic acids research · 2016Article
- CbbR, the Master Regulator for Microbial Carbon Dioxide Fixation.Journal of bacteriology · 2015Review
- Autotrophic carbon dioxide fixation via the Calvin-Benson-Bassham cycle by the denitrifying methanotroph "Candidatus Methylomirabilis oxyfera".Applied and environmental microbiology · 2014Article
- Three of four GlnR binding sites are essential for GlnR-mediated activation of transcription of the Amycolatopsis mediterranei nas operon.Journal of bacteriology · 2013Article
- Dual role of CcpC protein in regulation of aconitase gene expression in Listeria monocytogenes and Bacillus subtilis.Microbiology (Reading, England) · 2013Article
- Mycobacterium tuberculosis WhiB1 represses transcription of the essential chaperonin GroEL2.Tuberculosis (Edinburgh, Scotland) · 2012Article
- Further unraveling the regulatory twist by elucidating metabolic coinducer-mediated CbbR-cbbI promoter interactions in Rhodopseudomonas palustris CGA010.Journal of bacteriology · 2012Article
- Article
- Regulatory system of the protocatechuate 4,5-cleavage pathway genes essential for lignin downstream catabolism.Journal of bacteriology · 2010Article
- Expression and regulation of ribulose 1,5-bisphosphate carboxylase/oxygenase genes in Mycobacterium sp. strain JC1 DSM 3803.Journal of microbiology (Seoul, Korea) · 2009Article
- Atrazine biodegradation in the lab and in the field: enzymatic activities and gene regulation.Microbial biotechnology · 2009Review
- Molecular mechanism of the regulation of Bacillus subtilis gltAB expression by GltC.Journal of molecular biology · 2007Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The LysR-type transcriptional regulator CbbR controls the expression of the cbb and gap-pgk operons in Xanthobacter flavus, which encode the majority of the enzymes of the Calvin cycle required for autotrophic CO2 fixation. The cbb operon promoter of this chemoautotrophic bacterium contains three potential CbbR binding sites, two of which partially overlap. Site-directed mutagenesis and subsequent analysis of DNA binding by CbbR and cbb promoter activity were used to show that the potential CbbR binding sequences are functional. Inverted repeat IR1 is a high-affinity CbbR binding site. The main function of this repeat is to recruit CbbR to the cbb operon promoter. In addition, it is required for negative autoregulation of cbbR expression. IR3 represents the main low-affinity binding site of CbbR. Binding to IR3 occurs in a cooperative manner, since mutations preventing the binding of CbbR to IR1 also prevent binding to the low-affinity site. Although mutations in IR3 have a negative effect on the binding of CbbR to this site, they result in an increased promoter activity. This is most likely due to steric hindrance of RNA polymerase by CbbR since IR3 partially overlaps with the -35 region of the cbb operon promoter. Mutations in IR2 do not affect the DNA binding of CbbR in vitro but have a severe negative effect on the activity of the cbb operon promoter. This IR2 binding site is therefore critical for transcriptional activation by CbbR.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.