ArticleJournal of bacteriology2003
Effect of intracellular pH on rotational speed of bacterial flagellar motors.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
55 citing papers in PubMed.
- Oxygen-dependent partitioning of acetate assimilation via Acs and AckA-Pta pathways inJournal of bacteriology · 2026Article
- HilE mediates motility thermoregulation in typhoidal Salmonella serovars at elevated physiological temperatures.PLoS pathogens · 2025Article
- Species-specific surface dwell times and accumulation of microbes investigated by holographic microscopy.Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2025Article
- Breaking the reproducibility barrier with standardized protocols for plant-microbiome research.PLoS biology · 2025Article
- Bacterial Minicell-Based Biohybrid Sub-micron Swimmers for Targeted Cargo Delivery.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Intracellular formate modulates a motility-invasion switch in Salmonella Typhimurium.PLoS pathogens · 2025Article
- Combined response of polar magnetotaxis to oxygen and pH: Insights from hanging drop assays and microcosm experiments.Scientific reports · 2024Article
- Riboswitch and small RNAs modulate btuB translation initiation in Escherichia coli and trigger distinct mRNA regulatory mechanisms.Nucleic acids research · 2024Article
- Quantifying Substrate Protein Secretion via the Type III Secretion System of the Bacterial Flagellum.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Unraveling the Intertwined Effect of pH onMicroorganisms · 2023Article
- Lanthanide porphyrinoids as molecular theranostics.Chemical Society reviews · 2022Review
- pH Distribution along Growing Fungal Hyphae at Microscale.Journal of fungi (Basel, Switzerland) · 2022Article
- TheJournal of bacteriology · 2022Article
- Review
- The Dynamic Ion Motive Force Powering the Bacterial Flagellar Motor.Frontiers in microbiology · 2021Review
- LPS-binding IgG arrests actively motile Salmonella Typhimurium in gastrointestinal mucus.Mucosal immunology · 2020Article
- GFP Fusion to the N-Terminus of MotB Affects the Proton Channel Activity of the Bacterial Flagellar Motor inBiomolecules · 2020Article
- Coaxial nanofibers outperform uniaxial nanofibers for the loading and release of pyrroloquinoline quinone (PQQ) for biomedical applications.Nanoscale advances · 2020Article
- Spirochete Flagella and Motility.Biomolecules · 2020Review
- Flagella-Driven Motility of Bacteria.Biomolecules · 2019Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Weak acids such as acetate and benzoate, which partially collapse the transmembrane proton gradient, not only mediate pH taxis but also impair the motility of Escherichia coli and Salmonella at an external pH of 5.5. In this study, we examined in more detail the effect of weak acids on motility at various external pH values. A change of external pH over the range 5.0 to 7.8 hardly affected the swimming speed of E. coli cells in the absence of 34 mM potassium acetate. In contrast, the cells decreased their swimming speed significantly as external pH was shifted from pH 7.0 to 5.0 in the presence of 34 mM acetate. The total proton motive force of E. coli cells was not changed greatly by the presence of acetate. We measured the rotational rate of tethered E. coli cells as a function of external pH. Rotational speed decreased rapidly as the external pH was decreased, and at pH 5.0, the motor stopped completely. When the external pH was returned to 7.0, the motor restarted rotating at almost its original level, indicating that high intracellular proton (H+) concentration does not irreversibly abolish flagellar motor function. Both the swimming speeds and rotation rates of tethered cells of Salmonella also decreased considerably when the external pH was shifted from pH 7.0 to 5.5 in the presence of 20 mM benzoate. We propose that the increase in the intracellular proton concentration interferes with the release of protons from the torque-generating units, resulting in slowing or stopping of the motors.
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