ArticleJournal of bacteriology2003
YfiK from Escherichia coli promotes export of O-acetylserine and cysteine.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed.
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- O-Acetyl-Serine Supplementation Enhances Insulin Secretion and Improves Postprandial Glycaemia in Lean and Prediabetic Mice.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
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- Exchange of the L-cysteine exporter after in-vivo metabolic control analysis improved the L-cysteine production process with engineered Escherichia coli.Microbial cell factories · 2025Article
- Metabolic control analysis enabled the improvement of the L-cysteine production process with Escherichia coli.Applied microbiology and biotechnology · 2024Article
- Cyclodipeptide oxidase is an enzyme filament.Nature communications · 2024Article
- Regulation of Cysteine Homeostasis and Its Effect onInternational journal of molecular sciences · 2024Article
- Pseudomonas putida as saviour for troubled Synechococcus elongatus in a synthetic co-culture - interaction studies based on a multi-OMICs approach.Communications biology · 2024Article
- Challenges and Advances in the Bioproduction of L-Cysteine.Molecules (Basel, Switzerland) · 2024Review
- Stress-induced metabolic exchanges between complementary bacterial types underly a dynamic mechanism of inter-species stress resistance.Nature communications · 2023Article
- Microbial Production Potential ofMicroorganisms · 2022Review
- A Novel Efficient L-Lysine Exporter Identified by Functional Metagenomics.Frontiers in microbiology · 2022Article
- The Lrp/AsnC-Type Regulator PA2577 Controls the EamA-like Transporter GeneInternational journal of molecular sciences · 2021Article
- Cellular export of sugars and amino acids: role in feeding other cells and organisms.Plant physiology · 2021Review
- Biosynthesis and Heterologous Production of Mycosporine-Like Amino Acid Palythines.The Journal of organic chemistry · 2021Article
- Redox homeostasis in Mycobacterium tuberculosis is modulated by a novel actinomycete-specific transcription factor.The EMBO journal · 2021Article
- Identification and Characterization of an O-Succinyl-L-Homoserine Sulfhydrylase FromFrontiers in chemistry · 2021Article
- Metabolism and Regulatory Functions ofFrontiers in plant science · 2021Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
yfiK was discovered as a gene augmenting cysteine production when it was overexpressed in an industrial Escherichia coli production strain. The gene product is an integral membrane protein with about six predicted transmembrane helices; it belongs to the RhtB family of export proteins. YfiK overproduction from a plasmid leads to drastic and parallel secretion of O-acetylserine and cysteine into the medium but only when the organism possesses a serine transacetylase that is feedback insensitive to cysteine. Externally provided O-acetylserine obviated this requirement for cysteine secretion both in the yfiK-carrying transformant and in the wild type. A DeltayfiK mutant did not show any phenotype, and it exported O-acetylserine and cysteine when transformed with a plasmid carrying ydeD, a previously characterized, alternate O-acetylserine/cysteine exporter. Since a ydeD-yfiK double mutant showed the same pattern, it appears that YfiK and YdeD act independently. The necessity for the cell to regulate the size of the internal pool of O-acetylserine via synthesis of exporter proteins could be connected to the fact that this compound (when supplied externally) inhibits growth. Overexpression of either ydeD or yfiK leads to alleviation of this inhibition paralled by increased resistance to azaserine, which is an analog of O-acetylserine.
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