ArticleJournal of bacteriology2003
Molecular analysis of the gene encoding a novel cold-adapted chitinase (ChiB) from a marine bacterium, Alteromonas sp. strain O-7.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 40 citations in OpenAlex.
- Characterization of an Atypical GH19 Family Chitinase fromMicroorganisms · 2026Article
- Genomics, Proteomics, and Antifungal Activity of Chitinase from the Antarctic Marine BacteriumInternational journal of molecular sciences · 2024Article
- Microbial chitinases and their relevance in various industries.Folia microbiologica · 2023Review
- Identification and Characterization of Three Chitinases with Potential in Direct Conversion of Crystalline Chitin intoMarine drugs · 2022Article
- Extremophile - An Adaptive Strategy for Extreme Conditions and Applications.Current genomics · 2020Review
- Chitinase genes fromRoyal Society open science · 2019Article
- The stability of the TIM-barrel domain of a psychrophilic chitinase.Biochemistry and biophysics reports · 2015Article
- Cold shock induction of recombinant Arctic environmental genes.BMC biotechnology · 2015Article
- Psychrotolerant antifungal Streptomyces isolated from Tawang, India and the shift in chitinase gene family.Extremophiles : life under extreme conditions · 2013Article
- Sequence and structural investigation of a novel psychrophilic α-amylase from Glaciozyma antarctica PI12 for cold-adaptation analysis.Journal of molecular modeling · 2013Article
- Structural prediction of a novel chitinase from the psychrophilic Glaciozyma antarctica PI12 and an analysis of its structural properties and function.Journal of computer-aided molecular design · 2012Article
- Molecular cloning, expression and biochemical characterisation of a cold-adapted novel recombinant chitinase from Glaciozyma antarctica PI12.Microbial cell factories · 2011Article
- Cloning, characterization and expression of the chitinase gene of Enterobacter sp. NRG4.Indian journal of microbiology · 2008Article
- Molecular analysis of the gene encoding a new chitinase from the marine psychrophilic bacterium Moritella marina and biochemical characterization of the recombinant enzyme.Extremophiles : life under extreme conditions · 2008Article
- Isolation and characterization of a chitinase gene from entomopathogenic fungus Verticillium lecanii.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2008Article
- Family 18 chitolectins: comparison of MGP40 and HUMGP39.Biochemical and biophysical research communications · 2007Article
- Legionella pneumophila type II secretome reveals unique exoproteins and a chitinase that promotes bacterial persistence in the lung.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Isolation, characterization, and transcriptional analysis of the chitinase chi2 Gene (DQ011663) from the biocontrol fungus Metarhizium anisopliae var. anisopliae.Current microbiology · 2006Article
- Roles of four chitinases (chia, chib, chic, and chid) in the chitin degradation system of marine bacterium Alteromonas sp. strain O-7.Applied and environmental microbiology · 2005Article
- Cloning of Beauveria bassiana chitinase gene Bbchit1 and its application to improve fungal strain virulence.Applied and environmental microbiology · 2005Article
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8 authors at 1 institution in 1 country.
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Abstract
The chitinase B (ChiB) secreted by Alteromonas sp. strain O-7 was purified, and the corresponding gene (chiB) was cloned and sequenced. The open reading frame of the chiB gene encodes a protein of 850 amino acids with a calculated molecular mass of 90,223 Da. ChiB is a modular enzyme consisting of two reiterated domains and a catalytic domain belonging to chitinase family 18. The reiterated domains are composed of chitin-binding domain (ChtBD) type 3 and two fibronectin type III (Fn3)-like domains. Expression plasmids coding for ChiB or deletion derivatives thereof were constructed in Escherichia coli. Deletion analysis showed that the ChtBD of ChiB plays an important role in efficient hydrolysis of insoluble chitin. The optimum pH and temperature of ChiB were 6.0 and 30 degrees C, respectively. The enzyme showed relatively high catalysis, even at low temperatures close to 0 degrees C, and remarkable thermal lability compared to ChiA and ChiC, which are the mesophilic chitinases of the same strain. The kca)/Km value for the ChiB reaction at 10 degrees C was about 4.7 times higher than that of ChiC. These results suggest that ChiB is a cold-adapted enzyme. The RNA transcript of chiB was induced by 1% GlcNAc, and along with a rise in temperature, the RNA transcript showed a tendency to decrease. Thus, among the ChiA, ChiB, and ChiC chitinases, production of ChiB may be advantageous for the strain, allowing it to easily acquire nutrients from chitin and to survive in cold environments.
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