ArticleNucleic acids research2003
A mini-RNA containing the tetraloop, wobble-pair and loop E motifs of the central conserved region of potato spindle tuber viroid is processed into a minicircle.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 45 citations in OpenAlex.
- Derailing the host machinery to achieve replication: how viroid and viroid-like RNAs successfully copy their genomes in hostile territory.RNA biology · 2025Review
- Resistance of the CRISPR-Cas13a Gene-Editing System to Potato Spindle Tuber Viroid Infection in Tomato andViruses · 2024Article
- Viroids, Satellite RNAs and Prions: Folding of Nucleic Acids and Misfolding of Proteins.Viruses · 2024Article
- Conserved Motifs and Domains in Members ofCells · 2022Article
- Progress in 50 years of viroid research-Molecular structure, pathogenicity, and host adaptation.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2021Article
- Insights Into Potato Spindle Tuber Viroid Quasi-Species From Infection to Disease.Frontiers in microbiology · 2020Article
- In silico survey of the central conserved regions in viroids of theRNA (New York, N.Y.) · 2019Article
- Processing of Potato Spindle Tuber Viroid RNAs in Yeast, a Nonconventional Host.Journal of virology · 2017Article
- Modelling the three-dimensional structure of the right-terminal domain of pospiviroids.Scientific reports · 2017Article
- The RNA strands of the plus and minus polarities of peach latent mosaic viroid fold into different structures.RNA (New York, N.Y.) · 2010Article
- A genomic map of viroid RNA motifs critical for replication and systemic trafficking.The Plant cell · 2008Article
- Processing of nuclear viroids in vivo: an interplay between RNA conformations.PLoS pathogens · 2007Article
- Structural differences within the loop E motif imply alternative mechanisms of viroid processing.RNA (New York, N.Y.) · 2007Article
- Evidence for the existence of the loop E motif of Potato spindle tuber viroid in vivo.Journal of virology · 2007Article
- Tertiary structural and functional analyses of a viroid RNA motif by isostericity matrix and mutagenesis reveal its essential role in replication.Journal of virology · 2006Article
- Chrysanthemum stunt viroid disturbs the photoperiodic response for flowering of chrysanthemum plants.Planta · 2004Article
- Characterization of the RNA motif responsible for the specific interaction of potato spindle tuber viroid RNA (PSTVd) and the tomato protein Virp1.Nucleic acids research · 2003Article
- RNA conformational changes in the life cycles of RNA viruses, viroids, and virus-associated RNAs.Biochimica et biophysica actaReview
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3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A Mini-RNA from potato spindle tuber viroid (PSTVd) was constructed specifically for cleavage and ligation to circles in vitro. It contains the C-domain with the so-called central conserved region (CCR) of PSTVd with a 17 nt duplication in the upper strand and hairpin structures at the left and rights ends of the secondary structure. The CCR was previously shown to be essential for processing of in vitro transcripts. When folded under conditions which favor formation of a kinetically controlled conformation and incubated in a potato nuclear extract, the Mini-RNA is cleaved correctly at the 5'- and the 3'-end and ligated to a circle. Thus, the CCR obviously contains all structural and functional requirements for correct processing and therefore may be regarded as 'processing domain' of PSTVd. Using the Mini-RNA as a model substrate, the structural and functional relevance of its conserved non-canonical motifs GAAA tetraloop, loop E and G:U wobble base pair were studied by mutational analysis. It was found that (i) the conserved GAAA tetraloop is essential for processing by favoring the kinetically controlled conformation, (ii) a G:U wobble base pair at the 5'-cleavage site contributes to its correct recognition and (iii) an unpaired nucleotide in loop E, which is different from the corresponding nucleotide in the conserved loop E motif, is essential for ligation of the 5'- with the 3'-end. Hence all three structural motifs are functional elements for processing in a potato nuclear extract.
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