ArticleNucleic acids research2003
Sir2p suppresses recombination of replication forks stalled at the replication fork barrier of ribosomal DNA in Saccharomyces cerevisiae.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 38 citations in OpenAlex.
- Senescence in yeast is associated with amplified linear fragments of chromosome XII rather than ribosomal DNA circle accumulation.PLoS biology · 2023Article
- The abundance of Fob1 modulates the efficiency of rRFBs to stall replication forks.Nucleic acids research · 2017Article
- The Epigenetic Pathways to Ribosomal DNA Silencing.Microbiology and molecular biology reviews : MMBR · 2016Review
- Sumoylation of Sir2 differentially regulates transcriptional silencing in yeast.Nucleic acids research · 2015Article
- Expansion of Interstitial Telomeric Sequences in Yeast.Cell reports · 2015Article
- Mechanism of regulation of 'chromosome kissing' induced by Fob1 and its physiological significance.Genes & development · 2015Article
- Electrophoretic mobility of supercoiled, catenated and knotted DNA molecules.Nucleic acids research · 2015Article
- Mechanism and physiological significance of programmed replication termination.Seminars in cell & developmental biology · 2014Review
- Topoisomerase 2 is dispensable for the replication and segregation of small yeast artificial chromosomes (YACs).PloS one · 2014Article
- Repeat expansion in the budding yeast ribosomal DNA can occur independently of the canonical homologous recombination machinery.Nucleic acids research · 2011Article
- Replicative age induces mitotic recombination in the ribosomal RNA gene cluster of Saccharomyces cerevisiae.PLoS genetics · 2011Article
- Contrasting roles of checkpoint proteins as recombination modulators at Fob1-Ter complexes with or without fork arrest.Eukaryotic cell · 2009Article
- Replication fork barriers: pausing for a break or stalling for time?EMBO reports · 2007Review
- The mitochondrial transcription termination factor mTERF modulates replication pausing in human mitochondrial DNA.Nucleic acids research · 2007Article
- CTD kinase I is required for the integrity of the rDNA tandem array.Nucleic acids research · 2006Article
- Chromosome XII context is important for rDNA function in yeast.Nucleic acids research · 2006Article
- The mating type switch-activating protein Sap1 Is required for replication fork arrest at the rRNA genes of fission yeast.Molecular and cellular biology · 2005Article
- Article
- Transcription termination factor reb1p causes two replication fork barriers at its cognate sites in fission yeast ribosomal DNA in vivo.Molecular and cellular biology · 2004Article
- Plasmid accumulation reduces life span in Saccharomyces cerevisiae.The Journal of biological chemistry · 2003Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the ribosomal DNA (rDNA) of Saccharomyces cerevisiae replication forks progressing against transcription stall at a polar replication fork barrier (RFB) located close to and downstream of the 35S transcription unit. Forks blocked at this barrier are potentially recombinogenic. Plasmids bearing the RFB sequence in its active orientation integrated into the chromosomal rDNA in sir2 mutant cells but not in wild-type cells, indicating that the histone deacetylase silencing protein Sir2 (Sir2p), which also modulates the aging process in yeast, suppresses the recombination competence of forks blocked at the rDNA RFB. Orientation of the RFB sequence in its inactive course or its abolition by FOB1 deletion avoided plasmid integration in sir2 mutant cells, indicating that stalling of the forks in the plasmid context was required for recombination to take place. Altogether these results strongly suggest that one of the functions of Sir2p is to modulate access of the recombination machinery to the forks stalled at the rDNA RFB.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.