ArticleMolecular pathology : MP2003
Effect of Helicobacter pylori on apoptosis and apoptosis related genes in gastric cancer cells.
Article in Molecular pathology : MP, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 48 citations in OpenAlex.
- Significant Association ofBritish journal of biomedical science · 2022Article
- Analysis of Gene Expression of miRNA-106b-5p andGenes · 2020Article
- Alterations of the TP53 gene in gastric and esophageal carcinogenesis.Journal of biomedicine & biotechnology · 2012Review
- Nuclear translocation of β-catenin correlates with CD44 upregulation in Helicobacter pylori-infected gastric carcinoma.Molecular and cellular biochemistry · 2011Article
- Differential expression of MYC in H. pylori-related intestinal and diffuse gastric tumors.Virchows Archiv : an international journal of pathology · 2011Article
- The role of oncogenes in gastrointestinal cancer.Gastrointestinal cancer research : GCR · 2010Article
- HOGG1 polymorphism in atrophic gastritis and gastric cancer after Helicobacter pylori eradication.World journal of gastroenterology · 2010Article
- Relationship between H.Pylori infection and clinicopathological features and prognosis of gastric cancer.BMC cancer · 2010Article
- Identification of novel cyclooxygenase-2-dependent genes in Helicobacter pylori infection in vivo.Molecular cancer · 2009Article
- Helicobacter pylori suppresses glycogen synthase kinase 3beta to promote beta-catenin activity.The Journal of biological chemistry · 2008Article
- MYC and gastric adenocarcinoma carcinogenesis.World journal of gastroenterology · 2008Review
- H pylori (CagA) and Epstein-Barr virus infection in gastric carcinomas: correlation with p53 mutation and c-Myc, Bcl-2 and Bax expression.World journal of gastroenterology · 2008Article
- Helicobacter pylori eradication for gastric cancer prevention.Journal of gastroenterology · 2007Review
- Detection of H. pylori antibody profile in serum by protein array.World journal of gastroenterology · 2006Article
- Activation of beta-catenin by carcinogenic Helicobacter pylori.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Expression of bcl-2 oncogene in gastric precancerous lesions and its correlation with syndromes in traditional Chinese medicine.World journal of gastroenterology · 2005Article
- Relationship of Helicobacter pylori to Bcl-2 family expression, DNA content, and pathological characteristics of gastric cancer.International journal of gastrointestinal cancer · 2005Article
- Expression of MTLC gene in gastric carcinoma.World journal of gastroenterology · 2003Article
- Identification of H. pylori strain specific DNA sequences between two clinical isolates from NUD and gastric ulcer by SSH.World journal of gastroenterology · 2003Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND/
aimsHelicobacter pylori induces the apoptosis of gastric epithelial cells in vivo and in vitro. However, the molecular mechanism has not been clarified. The aim of this study was to investigate the effect of H pylori on the apoptosis of gastric epithelial cells and the expression of apoptosis related genes in vitro.
methodsHuman gastric adenocarcinoma SGC-7901 cells were co-cultured with a cytotoxic H pylori strain, NCTC 11637, at various densities ranging from 3.2 x 10(4) to 1.0 x 10(8) colony forming units (CFU)/ml for 48 hours. Apoptosis in gastric cells was determined by transmission electron microscopy, Hoechst 33258 fluorochrome staining, and flow cytometry. The expression of apoptosis related proteins, Bcl-2, Bax, and c-Myc, was measured by an immunohistochemical method, and c-Myc mRNA expression was determined by the reverse transcription-polymerase chain reaction.
resultsHelicobacter pylori induces morphological changes typical of apoptosis. Both fluorochrome staining and flow cytometry showed that the apoptotic index began to increase when H pylori were at a density of > 1.6 x 10(4) CFU/ml, and in a density dependent manner (p < 0.01; one way ANOVA). The expression of the Bax and c-Myc proteins and of c-Myc mRNA was increased, whereas Bcl-2 expression was decreased after co-culture for 48 hours.
conclusionsHelicobacter pylori induced apoptosis in gastric epithelial cells is mediated by altered expression of the products of the Bcl-2, Bax, and c-Myc genes.
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