ArticleThe Biochemical journal2003
Troponin C in different insect muscle types: identification of two isoforms in Lethocerus, Drosophila and Anopheles that are specific to asynchronous flight muscle in the adult insect.
Article in The Biochemical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 76 citations in OpenAlex.
- Fine-tuning striated muscle performance: conserved sarcomere-level mechanisms across insect and vertebrate systems.Frontiers in physiology · 2026Review
- Parvin is essential for indirect flight muscle (IFM) performance and Zasp52 localisation to the Z-disc in Drosophila melanogaster.Cell and tissue research · 2025Article
- Immune system modulation & virus transmission during parasitism identified by multi-species transcriptomics of a declining insect biocontrol system.BMC genomics · 2024Article
- Identification of evolutionarily conserved regulators of muscle mitochondrial network organization.Nature communications · 2022Article
- Mitochondrial network configuration influences sarcomere and myosin filament structure in striated muscles.Nature communications · 2022Article
- Mandibular muscle troponin of the Florida carpenter ant Camponotus floridanus: extending our insights into invertebrate CaJournal of muscle research and cell motility · 2021Article
- Review
- Nanoscopy reveals the layered organization of the sarcomeric H-zone and I-band complexes.The Journal of cell biology · 2020Article
- Stretch activation properties of Drosophila and Lethocerus indirect flight muscle suggest similar calcium-dependent mechanisms.American journal of physiology. Cell physiology · 2017Article
- Functional redundancy and nonredundancy between two Troponin C isoforms inMolecular biology of the cell · 2017Article
- Transcriptome Comparison Analysis of Ostrinia furnacalis in Four Developmental Stages.Scientific reports · 2016Article
- An undergraduate laboratory class using CRISPR/Cas9 technology to mutate drosophila genes.Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology · 2016Article
- Article
- Expression of the Troponin C at 41C Gene in Adult Drosophila Tubular Muscles Depends upon Both Positive and Negative Regulatory Inputs.PloS one · 2015Article
- The roles of troponin C isoforms in the mechanical function of Drosophila indirect flight muscle.Journal of muscle research and cell motility · 2014Article
- Roles of the troponin isoforms during indirect flight muscle development in Drosophila.Journal of genetics · 2014Article
- An embryonic myosin converter domain influences Drosophila indirect flight muscle stretch activation, power generation and flight.The Journal of experimental biology · 2014Article
- Mutations of the Drosophila myosin regulatory light chain affect courtship song and reduce reproductive success.PloS one · 2014Article
- Regulating the contraction of insect flight muscle.Journal of muscle research and cell motility · 2011Review
- Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The indirect flight muscles (IFMs) of Lethocerus (giant water bug) and Drosophila (fruitfly) are asynchronous: oscillatory contractions are produced by periodic stretches in the presence of a Ca(2+) concentration that does not fully activate the muscle. The troponin complex on thin filaments regulates contraction in striated muscle. The complex in IFM has subunits that are specific to this muscle type, and stretch activation may act through troponin. Lethocerus and Drosophila have an unusual isoform of the Ca(2+)-binding subunit of troponin, troponin C (TnC), with a single Ca(2+)-binding site near the C-terminus (domain IV); this isoform is only in IFMs, together with a minor isoform with an additional Ca(2+)-binding site in the N-terminal region (domain II). Lethocerus has another TnC isoform in leg muscle which also has two Ca(2+)-binding sites. Ca(2+) binds more strongly to domain IV than to domain II in two-site isoforms. There are four isoforms in Drosophila and Anopheles (malarial mosquito), three of which are also in adult Lethocerus. A larval isoform has not been identified in Lethocerus. Different TnC isoforms are expressed in the embryonic, larval, pupal and adult stages of Drosophila; the expression of the two IFM isoforms is increased in the pupal stage. Immunoelectron microscopy shows the distribution of the major IFM isoform with one Ca(2+)-binding site is uniform along Lethocerus thin filaments. We suggest that initial activation of IFM is by Ca(2+) binding to troponin with the two-site TnC, and full activation is through the action of stretch on the complex with the one-site isoform.
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