ArticleMolecular and cellular biology2003
Maintenance of open chromatin and selective genomic occupancy at the cell cycle-regulated histone H4 promoter during differentiation of HL-60 promyelocytic leukemia cells.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Epigenetic-Mediated Regulation of Gene Expression for Biological Control and Cancer: Cell and Tissue Structure, Function, and Phenotype.Results and problems in cell differentiation · 2022Review
- Higher order genomic organization and regulatory compartmentalization for cell cycle control at the G1/S-phase transition.Journal of cellular physiology · 2018Review
- A novel role for the Pol I transcription factor UBTF in maintaining genome stability through the regulation of highly transcribed Pol II genes.Genome research · 2015Article
- Genomic occupancy of HLH, AP1 and Runx2 motifs within a nuclease sensitive site of the Runx2 gene.Journal of cellular physiology · 2013Article
- The abbreviated pluripotent cell cycle.Journal of cellular physiology · 2013Review
- Epigenetic control of cell cycle-dependent histone gene expression is a principal component of the abbreviated pluripotent cell cycle.Molecular and cellular biology · 2012Article
- The architectural organization of human stem cell cycle regulatory machinery.Current pharmaceutical design · 2012Review
- Article
- Transcriptional corepressor TLE1 functions with Runx2 in epigenetic repression of ribosomal RNA genes.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- The histone gene activator HINFP is a nonredundant cyclin E/CDK2 effector during early embryonic cell cycles.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- The osteogenic transcription factor runx2 controls genes involved in sterol/steroid metabolism, including CYP11A1 in osteoblasts.Molecular endocrinology (Baltimore, Md.) · 2009Article
- G1 to S phase cell cycle transition in somatic and embryonic stem cells.Journal of anatomy · 2008Review
- Auranofin promotes retinoic acid- or dihydroxyvitamin D3-mediated cell differentiation of promyelocytic leukaemia cells by increasing histone acetylation.British journal of pharmacology · 2008Article
- Article
- Mitotic retention of gene expression patterns by the cell fate-determining transcription factor Runx2.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- HiNF-P directly links the cyclin E/CDK2/p220NPAT pathway to histone H4 gene regulation at the G1/S phase cell cycle transition.Molecular and cellular biology · 2005Article
- Identification of HiNF-P, a key activator of cell cycle-controlled histone H4 genes at the onset of S phase.Molecular and cellular biology · 2003Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
During the shutdown of proliferation and onset of differentiation of HL-60 promyelocytic leukemia cells, expression of the cell cycle-dependent histone genes is downregulated at the level of transcription. To address the mechanism by which this regulation occurs, we examined the chromatin structure of the histone H4/n (FO108, H4FN) gene locus. Micrococcal nuclease, DNase I, and restriction enzymes show similar cleavage sites and levels of sensitivity at the H4/n locus in both proliferating and differentiated HL-60 cells. In contrast, differentiation-related activation of the cyclin-dependent kinase inhibitor p21(cip1/WAF1) gene is accompanied by increased nuclease hypersensitivity. Chromatin immunoprecipitation assays of the H4/n gene reveal that acetylated histones H3 and H4 are maintained at the same levels in proliferating and postproliferative cells. Thus, the chromatin of the H4/n locus remains in an open state even after transcription ceases. Using ligation-mediated PCR to visualize genomic DNase I footprints at single-nucleotide resolution, we find that protein occupancy at the site II cell cycle element is selectively diminished in differentiated cells while the site I element remains occupied. Decreased occupancy of site II is reflected by loss of the site II binding protein HiNF-P. We conclude that H4 gene transcription during differentiation is downregulated by modulating protein interaction at the site II cell cycle element and that retention of an open chromatin conformation may be associated with site I occupancy.
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