ArticleMolecular and cellular biology2003
Loss of HR6B ubiquitin-conjugating activity results in damaged synaptonemal complex structure and increased crossing-over frequency during the male meiotic prophase.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 106 citations in OpenAlex.
- SUMO mediates the coordinate regulation of meiotic chromosome length and crossover rate.bioRxiv : the preprint server for biology · 2026Article
- Crosstalk Between Oxidative Stress and Epigenetics: Unveiling New Biomarkers in Human Infertility.Cells · 2024Review
- The role of ubiquitin-conjugating enzyme in the process of spermatogenesis.Reproductive biology and endocrinology : RB&E · 2024Review
- Epigenetic markers in the embryonal germ cell development and spermatogenesis.Basic and clinical andrology · 2023Review
- The ubiquitin-proteasome system regulates meiotic chromosome organization.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Histone Post-Translational Modifications and CircRNAs in Mouse and Human Spermatozoa: Potential Epigenetic Marks to Assess Human Sperm Quality.Journal of clinical medicine · 2020Review
- Sperm DNA Integrity and Male Fertility in Farm Animals: A Review.Frontiers in veterinary science · 2020Review
- Interplay between Caspase 9 and X-linked Inhibitor of Apoptosis Protein (XIAP) in the oocyte elimination during fetal mouse development.Cell death & disease · 2019Article
- DNA damage tolerance in stem cells, ageing, mutagenesis, disease and cancer therapy.Nucleic acids research · 2019Review
- Live cell analyses of synaptonemal complex dynamics and chromosome movements in cultured mouse testis tubules and embryonic ovaries.Chromosoma · 2018Article
- A functional variant in the UBE2B gene promoter is associated with idiopathic azoospermia.Reproductive biology and endocrinology : RB&E · 2015Article
- Article
- A universal method for sequential immunofluorescent analysis of chromatin and chromatin-associated proteins on chromosome spreads.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2013Article
- UBE2B mRNA alterations are associated with severe oligozoospermia in infertile men.Molecular human reproduction · 2013Article
- RNF168 forms a functional complex with RAD6 during the DNA damage response.Journal of cell science · 2013Article
- Sex chromosome inactivation in germ cells: emerging roles of DNA damage response pathways.Cellular and molecular life sciences : CMLS · 2012Review
- RAD6 regulates the dosage of p53 by a combination of transcriptional and posttranscriptional mechanisms.Molecular and cellular biology · 2012Article
- Membrane-associated RING-CH 10 (MARCH10 protein) is a microtubule-associated E3 ubiquitin ligase of the spermatid flagella.The Journal of biological chemistry · 2011Article
- Review
- Meiotic functions of RAD18.Journal of cell science · 2011Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ubiquitin-conjugating enzymes HR6A and HR6B are the two mammalian homologs of Saccharomyces cerevisiae RAD6. In yeast, RAD6 plays an important role in postreplication DNA repair and in sporulation. HR6B knockout mice are viable, but spermatogenesis is markedly affected during postmeiotic steps, leading to male infertility. In the present study, increased apoptosis of HR6B knockout primary spermatocytes was detected during the first wave of spermatogenesis, indicating that HR6B performs a primary role during the meiotic prophase. Detailed analysis of HR6B knockout pachytene nuclei showed major changes in the synaptonemal complexes. These complexes were found to be longer. In addition, we often found depletion of synaptonemal complex proteins from near telomeric regions in the HR6B knockout pachytene nuclei. Finally, we detected an increased number of foci containing the mismatch DNA repair protein MLH1 in these nuclei, reflecting a remarkable and consistent increase (20 to 25%) in crossing-over frequency. The present findings reveal a specific requirement for the ubiquitin-conjugating activity of HR6B in relation to dynamic aspects of the synaptonemal complex and meiotic recombination in spermatocytes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.