ArticleMolecular and cellular biology2003
Effect of the pheromone-responsive G(alpha) and phosphatase proteins of Saccharomyces cerevisiae on the subcellular localization of the Fus3 mitogen-activated protein kinase.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 49 citations in OpenAlex.
- Review
- Global quantitative understanding of non-equilibrium cell fate decision-making in response to pheromone.iScience · 2023Article
- Quantifying nonequilibrium dynamics and thermodynamics of cell fate decision making in yeast under pheromone induction.Biophysics reviews · 2023Article
- MAPK docking motif in the Dictyostelium Gα2 subunit is required for aggregation and transcription factor translocation.Cellular signalling · 2021Article
- Identification of a key G-protein coupled receptor in mediating appressorium formation and fungal virulence against insects.Science China. Life sciences · 2021Article
- Negative feedback-loop mechanisms regulating HOG- and pheromone-MAPK signaling in yeast.Current genetics · 2020Review
- Mitogen-Activated Protein Kinase Phosphatases (MKPs) in Fungal Signaling: Conservation, Function, and Regulation.International journal of molecular sciences · 2019Review
- MAP Kinase Regulation of themSphere · 2019Article
- Intracellular mechanism by which genotoxic stress activates yeast SAPK Mpk1.Molecular biology of the cell · 2018Article
- Recovery of novel association loci in Arabidopsis thaliana and Drosophila melanogaster through leveraging INDELs association and integrated burden test.PLoS genetics · 2018Article
- Single-cell dynamics and variability of MAPK activity in a yeast differentiation pathway.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Gβ promotes pheromone receptor polarization and yeast chemotropism by inhibiting receptor phosphorylation.Science signaling · 2016Article
- Compartmentalization of a bistable switch enables memory to cross a feedback-driven transition.Cell · 2015Article
- The Dictyostelium MAPK ERK1 is phosphorylated in a secondary response to early developmental signaling.Cellular signalling · 2015Article
- Specific α-arrestins negatively regulate Saccharomyces cerevisiae pheromone response by down-modulating the G-protein-coupled receptor Ste2.Molecular and cellular biology · 2014Article
- Network-dosage compensation topologies as recurrent network motifs in natural gene networks.BMC systems biology · 2014Article
- Formation of subnuclear foci is a unique spatial behavior of mating MAPKs during hyperosmotic stress.Cell reports · 2013Article
- A mechanism for the coordination of proliferation and differentiation by spatial regulation of Fus2p in budding yeast.Genes & development · 2012Article
- Cell-to-Cell Communication Circuits: Quantitative Analysis of Synthetic Logic Gates.Frontiers in physiology · 2012Article
- Pathway detection from protein interaction networks and gene expression data using color-coding methods and A∗ search algorithms.TheScientificWorldJournal · 2012Article
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6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mating-specific G(alpha) protein of Saccharomyces cerevisiae, Gpa1, stimulates adaptation to pheromone by a mechanism independent of G(beta gamma) sequestration. Genetic evidence suggests that Gpa1 targets the Fus3 mitogen-activated protein kinase, and it has recently been shown that the two proteins interact in cells responding to pheromone. To test the possibility that Gpa1 downregulates the mating signal by affecting the localization of Fus3, we created a Fus3-green fluorescent protein (GFP) fusion protein. In vegetative cells, Fus3-GFP was found in both the cytoplasm and the nucleus. Pheromone stimulated a measurable increase in the ratio of nuclear to cytoplasmic Fus3-GFP. In contrast, the relative level of nuclear Fus3-GFP decreased as cells recovered from pheromone arrest and did not increase when cells adapted to chronic stimulus were challenged again. Accumulation of Fus3-GFP in the nuclei of stimulated cells was also inhibited by overexpression of either wild-type Gpa1, the E364K hyperadaptive mutant form of Gpa1, or the Msg5 dually specific phosphatase. The effects of Gpa1 and Msg5 on Fus3 are partially interdependent. In a genetic screen for adaptive defective mutants, a nonsense allele of the nucleocytoplasmic transport receptor, Kap104, was identified. Truncation of the Kap104 cargo-binding domain blocked the effect of both Gpa1(E364K) and Msg5 on Fus3-GFP localization. Based on these results, we propose that Gpa1 and Msg5 work in concert to downregulate the mating signal and that they do so by inhibiting the pheromone-induced increase of Fus3 in the nucleus. Kap104 is required for the G(alpha)/phosphatase-mediated effect on Fus3 localization.
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