ArticleMolecular and cellular biology2003
The KH-domain protein alpha CP has a direct role in mRNA stabilization independent of its cognate binding site.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 44 citations in OpenAlex.
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- Efficient translation of Eggplant mottled dwarf nucleorhabdovirus N and X genes requires both 5' and 3' UTRs.Virology journal · 2021Article
- A cytosine-rich splice regulatory determinant enforces functional processing of the humanBlood · 2019Article
- Full-length mRNA sequencing uncovers a widespread coupling between transcription initiation and mRNA processing.Genome biology · 2018Article
- Article
- The Poly(C) Binding Protein Pcbp2 and Its Retrotransposed Derivative Pcbp1 Are Independently Essential to Mouse Development.Molecular and cellular biology · 2016Article
- Resistance of mRNAs with AUG-proximal nonsense mutations to nonsense-mediated decay reflects variables of mRNA structure and translational activity.Nucleic acids research · 2015Article
- Cytoplasmic poly(A) binding protein C4 serves a critical role in erythroid differentiation.Molecular and cellular biology · 2014Article
- Trypanosome MKT1 and the RNA-binding protein ZC3H11: interactions and potential roles in post-transcriptional regulatory networks.Nucleic acids research · 2014Article
- αCP Poly(C) binding proteins act as global regulators of alternative polyadenylation.Molecular and cellular biology · 2013Article
- Interaction of PABPC1 with the translation initiation complex is critical to the NMD resistance of AUG-proximal nonsense mutations.Nucleic acids research · 2012Article
- AUF-1 and YB-1 are critical determinants of β-globin mRNA expression in erythroid cells.Blood · 2012Article
- Article
- Control of human beta-globin mRNA stability and its impact on beta-thalassemia phenotype.Haematologica · 2011Review
- An RNA-protein complex links enhanced nuclear 3' processing with cytoplasmic mRNA stabilization.The EMBO journal · 2011Article
- The poly(rC)-binding protein alphaCP2 is a noncanonical factor in X. laevis cytoplasmic polyadenylation.RNA (New York, N.Y.) · 2011Article
- PCBP2 enhances the antiviral activity of IFN-α against HCV by stabilizing the mRNA of STAT1 and STAT2.PloS one · 2011Article
- Novel recognition motifs and biological functions of the RNA-binding protein HuD revealed by genome-wide identification of its targets.Nucleic acids research · 2010Article
- An iron responsive element-like stem-loop regulates alpha-hemoglobin-stabilizing protein mRNA.The Journal of biological chemistry · 2008Article
- Protein-RNA tethering: the role of poly(C) binding protein 2 in poliovirus RNA replication.Virology · 2008Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Previous studies suggest that high-level stability of a subset of mammalian mRNAs is linked to a C-rich motif in the 3' untranslated region (3'UTR). High-level expression of human alpha-globin mRNA (h alpha-globin mRNA) in erythroid cells has been specifically attributed to formation of an RNA-protein complex comprised of a 3'UTR C-rich motif and an associated 39-kDa poly(C) binding protein, alpha CP. Documentation of this RNA-protein alpha-complex has been limited to in vitro binding studies, and its impact has been monitored by alterations in steady-state mRNA. Here we demonstrate that alpha CP is stably bound to h alpha-globin mRNA in vivo, that alpha-complex assembly on the h alpha-globin mRNA is restricted to the 3'UTR C-rich motif, and that alpha-complex assembly extends the physical half-life of h alpha-globin mRNA selectively in erythroid cells. Significantly, these studies also reveal that an artificially tethered alpha CP has the same mRNA-stabilizing activity as the native alpha-complex. These data demonstrate a unique contribution of the alpha-complex to h alpha-globin mRNA stability and support a model in which the sole function of the C-rich motif is to selectively tether alpha CP to a subset of mRNAs. Once bound, alpha CP appears to be fully sufficient to trigger downstream events in the stabilization pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.