ArticleThe Biochemical journal2003
The internal alternative NADH dehydrogenase of Neurospora crassa mitochondria.
Article in The Biochemical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 41 citations in OpenAlex.
- Article
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- Supernatant of platelet-Microbiology spectrum · 2024Article
- Physiological relevance, localization and substrate specificity of the alternative (type II) mitochondrial NADH dehydrogenases ofFrontiers in microbiology · 2024Article
- Platelets Inhibit Methicillin-Resistant Staphylococcus aureus by Inducing Hydroxyl Radical-Mediated Apoptosis-Like Cell Death.Microbiology spectrum · 2022Article
- Article
- Contribution of Complex I NADH Dehydrogenase to Respiratory Energy Coupling in Glucose-Grown Cultures ofApplied and environmental microbiology · 2020Article
- The Effect of Aspergillus Thermomutatus Chrysovirus 1 on the Biology of Three Aspergillus Species.Viruses · 2018Article
- Alternative mitochondrial respiratory chains from two crustaceans: Artemia franciscana nauplii and the white shrimp, Litopenaeus vannamei.Journal of bioenergetics and biomembranes · 2018Article
- Apoptosis-inducing Factor (AIF) and Its Family Member Protein, AMID, Are Rotenone-sensitive NADH:Ubiquinone Oxidoreductases (NDH-2).The Journal of biological chemistry · 2015Article
- Mitochondrial type II NAD(P)H dehydrogenases in fungal cell death.Microbial cell (Graz, Austria) · 2015Review
- Characterization of an internal type-II NADH dehydrogenase from Chlamydomonas reinhardtii mitochondria.Current genetics · 2012Article
- Article
- Increased resistance of complex I mutants to phytosphingosine-induced programmed cell death.The Journal of biological chemistry · 2008Article
- New insights into type II NAD(P)H:quinone oxidoreductases.Microbiology and molecular biology reviews : MMBR · 2004Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An open reading frame homologous with genes of non-proton-pumping NADH dehydrogenases was identified in the genome of Neurospora crassa. The 57 kDa NADH:ubiquinone oxidoreductase acts as internal (alternative) respiratory NADH dehydrogenase (NDI1) in the fungal mitochondria. The precursor polypeptide includes a pre-sequence of 31 amino acids, and the mature enzyme comprises one FAD molecule as a prosthetic group. It catalyses specifically the oxidation of NADH. Western blot analysis of fungal mitochondria fractionated with digitonin indicated that the protein is located at the inner face of the inner membrane of the organelle (internal enzyme). The corresponding gene was inactivated by the generation of repeat-induced point mutations. The respiratory activity of mitochondria from the resulting null-mutant ndi1 is almost fully inhibited by rotenone, an inhibitor of the proton-pumping complex I, when matrix-generated NADH is used as substrate. Although no effects of the NDI1 defect on vegetative growth and sexual differentiation were observed, the germination of both sexual and asexual ndi1 mutant spores is significantly delayed. Crosses between the ndi1 mutant strain and complex I-deficient mutants yielded no viable double mutants. Our data indicate: (i) that NDI1 represents the sole internal alternative NADH dehydrogenase of Neurospora mitochondria; (ii) that NDI1 and complex I are functionally complementary to each other; and (iii) that NDI1 is specially needed during spore germination.
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