ArticleMolecular endocrinology (Baltimore, Md.)2003
A PIT-1 homeodomain mutant blocks the intranuclear recruitment of the CCAAT/enhancer binding protein alpha required for prolactin gene transcription.
Article in Molecular endocrinology (Baltimore, Md.), 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 55 citations in OpenAlex.
- Dose-dependent dual role of PIT-1 (POU1F1) in somatolactotroph cell proliferation and apoptosis.PloS one · 2015Article
- Regulation of LH/FSH expression by secretoglobin 3A2 in the mouse pituitary gland.Cell and tissue research · 2014Article
- Unraveling transcription factor interactions with heterochromatin protein 1 using fluorescence lifetime imaging microscopy and fluorescence correlation spectroscopy.Journal of biomedical optics · 2013Article
- The many faces of C/EBPδ and their relevance for inflammation and cancer.International journal of biological sciences · 2013Review
- A distal modular enhancer complex acts to control pituitary- and nervous system-specific expression of the LHX3 regulatory gene.Molecular endocrinology (Baltimore, Md.) · 2012Article
- Investigating protein-protein interactions in living cells using fluorescence lifetime imaging microscopy.Nature protocols · 2011Article
- Single nucleotide polymorphisms in bovine PRL gene and their associations with milk production traits in Chinese Holsteins.Molecular biology reports · 2010Article
- The 26-amino acid beta-motif of the Pit-1beta transcription factor is a dominant and independent repressor domain.Molecular endocrinology (Baltimore, Md.) · 2009Article
- ICF, an immunodeficiency syndrome: DNA methyltransferase 3B involvement, chromosome anomalies, and gene dysregulation.Autoimmunity · 2008Review
- Characterization of spectral FRET imaging microscopy for monitoring nuclear protein interactions.Journal of microscopy · 2007Article
- Functional sequestration of transcription factor activity by repetitive DNA.The Journal of biological chemistry · 2007Article
- Dynamic interactions between Pit-1 and C/EBPalpha in the pituitary cell nucleus.Molecular and cellular biology · 2006Article
- Functional interactions with Pit-1 reorganize co-repressor complexes in the living cell nucleus.Journal of cell science · 2005Article
- Quantitative imaging of protein interactions in the cell nucleus.BioTechniques · 2005Review
- Computer-assisted image analysis protocol that quantitatively measures subnuclear protein organization in cell populations.BioTechniques · 2004Article
- Imaging the localized protein interactions between Pit-1 and the CCAAT/enhancer binding protein alpha in the living pituitary cell nucleus.Molecular endocrinology (Baltimore, Md.) · 2003Article
- Characterization of an orange acceptor fluorescent protein for sensitized spectral fluorescence resonance energy transfer microscopy using a white-light laser.Journal of biomedical opticsArticle
- Characterization of an improved donor fluorescent protein for Forster resonance energy transfer microscopy.Journal of biomedical opticsArticle
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The pituitary-specific homeodomain protein Pit-1 cooperates with other transcription factors, including CCAAT/enhancer binding protein alpha (C/EBPalpha), in the regulation of pituitary lactotrope gene transcription. Here, we correlate cooperative activation of prolactin (PRL) gene transcription by Pit-1 and C/EBPalpha with changes in the subnuclear localization of these factors in living pituitary cells. Transiently expressed C/EBPalpha induced PRL gene transcription in pituitary GHFT1-5 cells, whereas the coexpression of Pit-1 and C/EBPalpha in HeLa cells demonstrated their cooperativity at the PRL promoter. Individually expressed Pit-1 or C/EBPalpha, fused to color variants of fluorescent proteins, occupied different subnuclear compartments in living pituitary cells. When coexpressed, Pit-1 recruited C/EBPalpha from regions of transcriptionally quiescent centromeric heterochromatin to the nuclear regions occupied by Pit-1. The homeodomain region of Pit-1 was necessary for the recruitment of C/EBPalpha. A point mutation in the Pit-1 homeodomain associated with the syndrome of combined pituitary hormone deficiency in humans also failed to recruit C/EBPalpha. This Pit-1 mutant functioned as a dominant inhibitor of PRL gene transcription and, instead of recruiting C/EBPalpha, was itself recruited by C/EBPalpha to centromeric heterochromatin. Together our results suggest that the intranuclear positioning of these factors determines whether they activate or silence PRL promoter activity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.