ArticleThe EMBO journal2003
In vivo evidence for the prokaryotic model of extended codon-anticodon interaction in translation initiation.
Article in The EMBO journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 28 citations in OpenAlex.
- Ultradeep characterisation of translational sequence determinants refutes rare-codon hypothesis and unveils quadruplet base pairing of initiator tRNA and transcript.Nucleic acids research · 2023Article
- Initiation at AUGUG and GUGUG sequences can lead to translation of overlapping reading frames in E. coli.Nucleic acids research · 2023Article
- Mechanisms governing codon usage bias and the implications for protein expression in the chloroplast of Chlamydomonas reinhardtii.The Plant journal : for cell and molecular biology · 2022Review
- Article
- Overcoming Poor Transgene Expression in the Wild-TypeMicroorganisms · 2022Article
- Overview of tRNA Modifications in Chloroplasts.Microorganisms · 2022Article
- Chloroplast Translation: Structural and Functional Organization, Operational Control, and Regulation.The Plant cell · 2018Review
- Proximity of the start codon to a leaderless mRNA's 5' terminus is a strong positive determinant of ribosome binding and expression in Escherichia coli.Journal of bacteriology · 2010Article
- Follow the leader: preference for specific amino acids directly following the initial methionine in proteins of different organisms.Genomics, proteomics & bioinformatics · 2010Article
- Recognition and positioning of mRNA in the ribosome by tRNAs with expanded anticodons.Journal of molecular biology · 2006Article
- Highly expressed proteins have an increased frequency of alanine in the second amino acid position.BMC genomics · 2006Article
- Regulatory sequences of orthologous petD chloroplast mRNAs are highly specific among Chlamydomonas species.Plant molecular biology · 2006Article
- Translation of chloroplast psbD mRNA in Chlamydomonas is controlled by a secondary RNA structure blocking the AUG start codon.Nucleic acids research · 2006Article
- Development of novel types of plastid transformation vectors and evaluation of factors controlling expression.Transgenic research · 2005Article
- Multiple elements required for translation of plastid atpB mRNA lacking the Shine-Dalgarno sequence.Nucleic acids research · 2004Article
- Evolution of monoblepharidalean fungi based on complete mitochondrial genome sequences.Nucleic acids research · 2003Article
- Chloroplast translation regulation.Photosynthesis researchReview
Corrections and comments
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Initiation codon context is an important determinant of translation initiation rates in both prokaryotes and eukaryotes. Such sequences include the Shine- Dalgarno ribosome-binding site, as well as other motifs surrounding the initiation codon. One proposed interaction is between the base immediately preceding the initiation codon (-1 position) and the nucleotide 3' to the tRNAf(Met) anticodon, at position 37. Adenine is conserved at position 37, and a uridine at -1 has been shown in vitro to favor initiation. We have tested this model in vivo, by manipulating the chloroplast of the green alga Chlamydomonas reinhardtii, where the translational machinery is prokaryotic in nature. We show that translational defects imparted by mutations at the petA -1 position can be suppressed by compensatory mutations at position 37 of an ectopically expressed tRNA(fMet). The mutant tRNAs are fully aminoacylated and do not interfere with the translation of other proteins. Although this extended base pairing is not an absolute requirement for initiation, it may convey added specificity to transcripts carrying non-standard initiation codons, and/or preserve translational fidelity under certain stress conditions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.