ArticleThe EMBO journal2003
A conserved tyrosine in the neck of a fungal kinesin regulates the catalytic motor core.
Article in The EMBO journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
- Kinesin Motors in the Filamentous Basidiomycetes in Light of theJournal of fungi (Basel, Switzerland) · 2022Review
- Molecular basis of coiled-coil oligomerization-state specificity.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Dissection of kinesin's processivity.PloS one · 2009Article
- Genetically engineered block copolymers: influence of the length and structure of the coiled-coil blocks on hydrogel self-assembly.Pharmaceutical research · 2008Article
- Flexibility of the neck domain enhances Kinesin-1 motility under load.Biophysical journal · 2006Article
- Review: regulation mechanisms of Kinesin-1.Journal of muscle research and cell motility · 2006Review
- Back on track - on the role of the microtubule for kinesin motility and cellular function.Journal of muscle research and cell motility · 2006Review
- The E-hook of tubulin interacts with kinesin's head to increase processivity and speed.Biophysical journal · 2005Article
- The complex interplay between the neck and hinge domains in kinesin-1 dimerization and motor activity.Molecular biology of the cell · 2005Article
- An intramolecular interaction between the FHA domain and a coiled coil negatively regulates the kinesin motor KIF1A.The EMBO journal · 2004Article
- Breathing down the neck of Unc104.The Journal of cell biology · 2003Review
- Phosphorylation of mitotic kinesin-like protein 2 by polo-like kinase 1 is required for cytokinesis.The Journal of cell biology · 2003Article
- Single fungal kinesin motor molecules move processively along microtubules.Biophysical journal · 2003Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The neck domain of fungal conventional kinesins displays characteristic properties which are reflected in a specific sequence pattern. The exchange of the strictly conserved Tyr 362, not present in animals, into Lys, Cys or Phe leads to a failure to dimerize. The destabilizing effect is confirmed by a lower coiled-coil propensity of mutant peptides. Whereas the Phe substitution has only a structural effect, the Lys and Cys replacements lead to dramatic kinetic changes. The steady state ATPase is 4- to 7-fold accelerated, which may be due to a faster microtubule-stimulated ADP release rate. These data suggest that an inhibitory effect of the fungal neck domain on the motor core is mediated by direct interaction of the aromatic ring of Tyr 362 with the head, whereas the OH group is essential for dimerization. This is the first demonstration of a direct influence of the kinesin neck region in regulation of the catalytic activity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.