ArticleProceedings of the National Academy of Sciences of the United States of America2003
Determination of the transcriptome of Vibrio cholerae during intraintestinal growth and midexponential phase in vitro.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 125 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
125 citing papers in PubMed, 225 citations in OpenAlex.
- Phosphate Limitation Modulates Vibrio Cholerae Outer Membrane Vesicle Formation, Composition and Toxicity.Journal of extracellular biology · 2026Article
- Antibacterial action, proteolytic immunity, and in vivo activity of a Vibrio cholerae microcin.Cell host & microbe · 2024Article
- Genomic and metabolic insights into the first host-associated isolate ofMicrobiology spectrum · 2023Article
- Modulation of Host-Microbe Metabolism by Cholera Toxin.Infection and immunity · 2023Review
- Vibrio cholerae RND efflux systems: mediators of stress responses, colonization and pathogenesis.Frontiers in cellular and infection microbiology · 2023Review
- Intestinal colonization againstAIMS microbiology · 2023Review
- Genome-wide association studies reveal distinct genetic correlates and increased heritability of antimicrobial resistance inMicrobial genomics · 2022Article
- Simulated Docking Predicts Putative Channels for the Transport of Long-Chain Fatty Acids inBiomolecules · 2022Article
- Pathogenicity and virulence regulation ofVirulence · 2020Review
- Control ofMicroorganisms · 2020Review
- A Family of Viral Satellites Manipulates Invading Virus Gene Expression and Can Affect Cholera Toxin Mobilization.mSystems · 2020Article
- Structural insights into the mechanism of oxidative activation of heme-free H-NOX from Vibrio cholerae.The Biochemical journal · 2020Article
- Diguanylate Cyclases inFrontiers in cellular and infection microbiology · 2020Review
- Adaptation ofFrontiers in microbiology · 2020Review
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- Vesicles FromFrontiers in microbiology · 2019Article
- Experimental bacterial adaptation to the zebrafish gut reveals a primary role for immigration.PLoS biology · 2018Article
- Inter-replicon Gene Flow Contributes to Transcriptional Integration in theG3 (Bethesda, Md.) · 2018Article
- In vivo repressed genes ofProceedings of the National Academy of Sciences of the United States of America · 2018Article
- A Requirement for Global Transcription Factor Lrp in Licensing Replication ofFrontiers in microbiology · 2018Article
65 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Vibrio cholerae is the etiologic bacterial agent of cholera, a severe diarrheal disease endemic in much of the developing world. The V. cholerae genome contains 3,890 genes distributed between a large and a small chromosome. Although the large chromosome encodes the majority of recognizable gene products and virulence determinants, the small chromosome carries a disproportionate number of hypothetical genes. Thus, little is known about the role of the small chromosome in the biology of this organism or other Vibrio species. We have used the rabbit ileal loop model of V. cholerae infection to obtain in vivo-grown cells under near midexponential conditions in the small-intestinal environment. We compared the global transcriptional pattern of these in vivo-grown cells to those grown to midexponential phase in rich medium under aerobic conditions. Under both conditions, the genes showing the highest levels of expression reside primarily on the large chromosome. However, a shift occurs in vivo that results in many more small chromosomal genes being expressed during growth in the intestine. Our analysis further suggests that nutrient limitation (particularly iron) and anaerobiosis are major stresses experienced by V. cholerae during growth in the rabbit upper intestine. Finally, relative to in vitro growth, the intestinal environment significantly enhanced expression of several virulence genes, including those involved in phenotypes such as motility, chemotaxis, intestinal colonization, and toxin production.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.