ArticleThe Journal of molecular diagnostics : JMD2003
Development of real-time PCR assays for the quantitative detection of Epstein-Barr virus and cytomegalovirus, comparison of TaqMan probes, and molecular beacons.
Article in The Journal of molecular diagnostics : JMD, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 60 citations in OpenAlex.
- Pentostatin, Cyclophosphamide, and Rituximab Followed by Alemtuzumab for Relapsed or Refractory Chronic Lymphocytic Leukemia: A Phase 2 Trial of the ECOG-Acrin Cancer Research Group (E2903).Acta haematologica · 2019Trial
- Brain microbial populations in HIV/AIDS: α-proteobacteria predominate independent of host immune status.PloS one · 2013Trial
- Molecular point-of-care: technological evolution, clinical applications, and future perspectives.Frontiers in bioengineering and biotechnology · 2026Review
- Persistence of a Skewed Repertoire of NK Cells in People with HIV-1 on Long-Term Antiretroviral Therapy.Journal of immunology (Baltimore, Md. : 1950) · 2024Article
- Case Report: Non-negligible Epstein-Barr virus-associated posttransplant lymphoproliferative disorders in a lung transplant recipient.Frontiers in immunology · 2023Article
- Implementation and Validation of the Roche Light Cycler 480 96-Well Plate Platform as a Real-Time PCR Assay for the Quantitative Detection of Cytomegalovirus (CMV) in Clinical Specimens Using the Luminex MultiCode ASRs System.Medical sciences (Basel, Switzerland) · 2020Article
- Contrast of Real-Time Fluorescent PCR Methods for Detection ofMicroorganisms · 2019Article
- In-silico Design of DNA Oligonucleotides: Challenges and Approaches.Computational and structural biotechnology journal · 2019Review
- Article
- Clinical application of a cancer genomic profiling assay to guide precision medicine decisions.Personalized medicine · 2017Article
- Prevalence and risk factors for oral DNA tumor viruses in HIV-infected youth.Journal of medical virology · 2016Article
- Relationship between the number of cytomegalovirus in anterior chamber and severity of anterior segment inflammation.Japanese journal of ophthalmology · 2013Article
- Using Epstein-Barr viral load assays to diagnose, monitor, and prevent posttransplant lymphoproliferative disorder.Clinical microbiology reviews · 2010Review
- Development of TaqMan MGB fluorescent real-time PCR assay for the detection of anatid herpesvirus 1.Virology journal · 2009Article
- Article
- Laboratory assays for Epstein-Barr virus-related disease.The Journal of molecular diagnostics : JMD · 2008Review
- Validation of Roche LightCycler Epstein-Barr virus quantification reagents in a clinical laboratory setting.The Journal of molecular diagnostics : JMD · 2006Article
- Real-time PCR in clinical microbiology: applications for routine laboratory testing.Clinical microbiology reviews · 2006Review
- Faster quantitative real-time PCR protocols may lose sensitivity and show increased variability.Nucleic acids research · 2005Article
- Differential methylation of genes that regulate cytokine signaling in lymphoid and hematopoietic tumors.Oncogene · 2005Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human Epstein-Barr virus (EBV) and cytomegalovirus (CMV) can cause serious complications in immunocompromised patients. Rapid diagnosis of EBV and CMV infection is critical in the management of the disease so that anti-viral therapy can be started early. Here we describe the development of real-time PCR assays using TaqMan probes and molecular beacons and compare the performance of both assays with a well-established, validated, gel-based PCR method for the quantification of EBV and CMV in patients' samples. The TaqMan and molecular beacon assays were linear between 10 to 10(7) viral genomes/reaction. Both assays generated calibration curves with strong correlation and low intra-assay and interassay variation. Results of EBV and CMV viral load determination inpatient samples obtained by the gel-based and real-time PCR were very similar. The real-time PCR assays showed increases in viral load before clinical measures of viral disease and decreases in viral load during anti-viral therapy in two of six pediatric patients. The data indicate that these TaqMan and molecular beacon approaches are accurate, rapid, and reliable assays for the diagnosis and monitoring of EBV and CMV infections in patients.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.