ArticleJournal of virology2003
Baculovirus alkaline nuclease possesses a 5'-->3' exonuclease activity and associates with the DNA-binding protein LEF-3.
Article in Journal of virology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 62 citations in OpenAlex.
- Genomic characterization of a baculovirus isolate, Malacosoma neustria nucleopolyhedrovirus Eriogaster neogena isolate (ManeNPV-Er) from Mongolia.Virology journal · 2026Article
- Structural transitions during the cooperative assembly of baculovirus single-stranded DNA-binding protein on ssDNA.Nucleic acids research · 2022Article
- Review
- Nucleocapsid Assembly of Baculoviruses.Viruses · 2019Review
- Global Analysis of Baculovirus Autographa californica Multiple Nucleopolyhedrovirus Gene Expression in the Midgut of the Lepidopteran Host Trichoplusia ni.Journal of virology · 2018Article
- The genome sequence of Pseudoplusia includens single nucleopolyhedrovirus and an analysis of p26 gene evolution in the baculoviruses.BMC genomics · 2015Article
- Article
- Screening of candidate proteins interacting with IE-2 of Bombyx mori nucleopolyhedrovirus.Molecular biology reports · 2013Article
- Analysis of the autographa californica multiple nucleopolyhedrovirus overlapping gene pair lef3 and ac68 reveals that AC68 is a per os infectivity factor and that LEF3 is critical, but not essential, for virus replication.Journal of virology · 2012Article
- Analysis of the genome of the sexually transmitted insect virus Helicoverpa zea nudivirus 2.Viruses · 2012Article
- Functional characterization of an alkaline exonuclease and single strand annealing protein from the SXT genetic element of Vibrio cholerae.BMC molecular biology · 2011Article
- Baculovirus: molecular insights on their diversity and conservation.International journal of evolutionary biology · 2011Article
- Identification of a domain of the baculovirus Autographa californica multiple nucleopolyhedrovirus single-strand DNA-binding protein LEF-3 essential for viral DNA replication.Journal of virology · 2010Article
- Multigene expression of protein complexes by iterative modification of genomic Bacmid DNA.BMC molecular biology · 2009Article
- Isolation and characterization of the DNA-binding protein (DBP) of the Autographa californica multiple nucleopolyhedrovirus.Virology · 2008Article
- A baculovirus alkaline nuclease knockout construct produces fragmented DNA and aberrant capsids.Virology · 2007Article
- The baculoviruses occlusion-derived virus: virion structure and function.Advances in virus research · 2007Review
- vlf-1 deletion brought AcMNPV to defect in nucleocapsid formation.Virus genes · 2005Article
- Identification of domains in Autographa californica multiple nucleopolyhedrovirus late expression factor 3 required for nuclear transport of P143.Journal of virology · 2005Article
- Characterization of a baculovirus lacking the alkaline nuclease gene.Journal of virology · 2004Article
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Alkaline nuclease (AN) of the Autographa californica multiple-capsid nucleopolyhedrovirus (AcMNPV) (open reading frame 133) was expressed in recombinant baculovirus as a His(6)-tagged fusion and purified by sequential chromatography on Ni-NTA-agarose, DEAE-Toyopearl, and heparin-Sepharose. At all stages of purification, AcMNPV AN was found to copurify with a 44-kDa polypeptide which was identified as the baculovirus single-stranded DNA (ssDNA)-binding (SSB) protein, LEF-3. Sedimentation analysis in glycerol gradients of highly purified samples suggested that AN and LEF-3 are associated in a complex (designated *AN/L3), predominantly as heterodimers, although oligomeric forms containing both proteins were evident. In reactions with single- or double-stranded 62-mer oligonucleotides that were labeled with (32)P at the 5' or 3' ends, *AN/L3 carried out exonucleolytic hydrolysis of both substrates exclusively in a 5'-->3' direction. Saturation of ssDNA with an excess of LEF-3 prior to the addition of *AN/L3 resulted in a marked decrease in the rate of ssDNA hydrolysis. This suggests that excess LEF-3 may protect ssDNA from digestion by a AN-LEF-3 complex, thus regulating its activity in infected cells. The association of baculovirus AN with the viral SSB LEF-3 and the 5'-->3' exonuclease activity of this complex suggests that AN and LEF-3 may participate in homologous recombination of the baculovirus genome in a manner similar to that of exonuclease (Redalpha) and DNA-binding protein (Redbeta) of the Red-mediated homologous recombination system of bacteriophage lambda.
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Registered trials
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