ArticleBiophysical journal2003
Stepwise transition of the tetra-manganese complex of photosystem II to a binuclear Mn2(micro -O)2 complex in response to a temperature jump: a time-resolved structural investigation employing x-ray absorption spectroscopy.
Article in Biophysical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed.
- The OJIP Kinetics Analysis Reveals Differential Thermal Tolerance Responses in Photosystem II ofPlants (Basel, Switzerland) · 2026Article
- Light increases resistance of thylakoid membranes to thermal inactivation.Journal of plant research · 2024Article
- From manganese oxidation to water oxidation: assembly and evolution of the water-splitting complex in photosystem II.Photosynthesis research · 2022Review
- Photosystem II oxygen-evolving complex photoassembly displays an inverse H/D solvent isotope effect under chloride-limiting conditions.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- An Overview of Biomembrane Functions in Plant Responses to High-Temperature Stress.Frontiers in plant science · 2018Review
- Structural insights into the light-driven auto-assembly process of the water-oxidizing MneLife · 2017Article
- Frequently asked questions about chlorophyll fluorescence, the sequel.Photosynthesis research · 2017Review
- Nitric Oxide (NO) in Plant Heat Stress Tolerance: Current Knowledge and Perspectives.Frontiers in plant science · 2017Review
- Production of Reactive Oxygen Species by Photosystem II as a Response to Light and Temperature Stress.Frontiers in plant science · 2016Review
- Article
- Production of reactive oxygen species in decoupled, Ca(2+)-depleted PSII and their use in assigning a function to chloride on both sides of PSII.Photosynthesis research · 2013Article
- Alleviation of heat damage to photosystem II by nitric oxide in tall fescue.Photosynthesis research · 2013Article
- Article
- Role of chloride ion in hydroxyl radical production in photosystem II under heat stress: electron paramagnetic resonance spin-trapping study.Journal of bioenergetics and biomembranes · 2012Article
- O2 reactions at the six-iron active site (H-cluster) in [FeFe]-hydrogenase.The Journal of biological chemistry · 2011Article
- Quality control of photosystem II: reactive oxygen species are responsible for the damage to photosystem II under moderate heat stress.The Journal of biological chemistry · 2008Article
- Heat-induced changes in the EPR signal of tyrosine D (Y(D)OX) a possible role of Cytochrome b559.Journal of bioenergetics and biomembranes · 2008Article
- On the structure of the manganese complex of photosystem II: extended-range EXAFS data and specific atomic-resolution models for four S-states.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2008Article
- Time-resolved X-ray spectroscopy leads to an extension of the classical S-state cycle model of photosynthetic oxygen evolution.Photosynthesis research · 2007Article
- Specific loss of the extrinsic 18 KDa protein from photosystem II upon heating to 47 degrees C causes inactivation of oxygen evolution likely due to Ca release from the Mn-complex.Photosynthesis research · 2005Article
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5 authors.
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Abstract
In oxygenic photosynthesis, water is oxidized at a protein-cofactor complex comprising four Mn atoms and, presumably, one calcium. Using multilayers of Photosystem II membrane particles, we investigated the time course of the disassembly of the Mn complex initiated by a temperature jump from 25 degrees C to 47 degrees C and terminated by rapid cooling after distinct heating periods. We monitored polarographically the oxygen-evolution activity, the amount of the Y(D)(ox) radical and of released Mn(2+) by EPR spectroscopy, and the structure of the Mn complex by x-ray absorption spectroscopy (XAS, EXAFS). Using a novel approach to analyze time-resolved EXAFS data, we identify three distinct phases of the disassembly process: (1) Loss of the oxygen-evolution activity and reduction of Y(D)(ox) occur simultaneously (k(1) = 1.0 min(-1)). EXAFS spectra reveal the concomitant loss of an absorber-backscatterer interaction between heavy atoms separated by approximately 3.3 A, possibly related to Ca release. (2) Subsequently, two Mn(III) or Mn(IV) ions seemingly separated by approximately 2.7 A in the native complex are reduced to Mn(II) and released (k(2) = 0.18 min(-1)). The x-ray absorption spectroscopy data is highly suggestive that the two unreleased Mn ions form a di- micro -oxo bridged Mn(III)(2) complex. (3) Finally, the tightly-bound Mn(2)( micro -O)(2) unit is slowly reduced and released (k(3) = 0.014 min(-1)).
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