ArticleBiophysical journal2003
Permeant cations and blockers modulate pH gating of ROMK channels.
Article in Biophysical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- A mathematical model of the rat kidney. IV. Whole kidney response to hyperkalemia.American journal of physiology. Renal physiology · 2022Article
- Article
- Cesium-associated hypokalemia successfully treated with amiloride.Clinical kidney journal · 2015Article
- Direct injection of cell-free Kir1.1 protein into Xenopus oocytes replicates single-channel currents derived from Kir1.1 mRNA.Channels (Austin, Tex.) · 2015Article
- Inhibition of ROMK channels by low extracellular K+ and oxidative stress.American journal of physiology. Renal physiology · 2013Article
- Residues at the outer mouth of Kir1.1 determine K-dependent gating.Biophysical journal · 2012Article
- Structural correlates of selectivity and inactivation in potassium channels.Biochimica et biophysica acta · 2012Review
- Potassium-dependent activation of Kir4.2 K⁺ channels.The Journal of physiology · 2011Article
- Modulation of Kir1.1 inactivation by extracellular Ca and Mg.Biophysical journal · 2011Article
- An intersubunit salt bridge near the selectivity filter stabilizes the active state of Kir1.1.Biophysical journal · 2009Article
- External K activation of Kir1.1 depends on the pH gate.Biophysical journal · 2007Article
- Localization of the pH gate in Kir1.1 channels.Biophysical journal · 2006Article
- Role of conserved glycines in pH gating of Kir1.1 (ROMK).Biophysical journal · 2006Article
- Interactive domains between pore loops of the yeast K+ channel TOK1 associate with extracellular K+ sensitivity.The Biochemical journal · 2006Article
- Structural locus of the pH gate in the Kir1.1 inward rectifier channel.Biophysical journal · 2005Article
- Molecular diversity and regulation of renal potassium channels.Physiological reviews · 2005Review
- Functional characterization of a prokaryotic Kir channel.The Journal of biological chemistry · 2004Article
- Potassium-dependent slow inactivation of Kir1.1 (ROMK) channels.Biophysical journal · 2004Article
- Regulation of Kir channels by intracellular pH and extracellular K(+): mechanisms of coupling.The Journal of general physiology · 2004Article
- Kir4.1 K+ channels are regulated by external cations.Channels (Austin, Tex.)Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
External potassium (K) activates the inward rectifier ROMK (K(ir)1.1) by altering the pH gating of the channel. The present study examines this link between external K and internal pH sensitivity using both the two-electrode voltage clamp and the perfused, cut-open Xenopus oocyte preparation. Elevating extracellular K from 1 mM to 10 mM to 100 mM activated ROMK channels by shifting their apparent pK(a) from 7.2 +/- 0.1 (n = 6) in 1 mM K, to 6.9 +/- 0.02 (n = 5) in 10 mM K, and to 6.6 +/- 0.03 (n = 5) in 100 mM K. At any given internal pH, the number of active ROMK channels is a saturating function of external [K]. Extracellular Cs (which blocks almost all inward K current) also stimulated outward ROMK conductance (at constant 1 mM external K) by shifting the apparent pK(a) of ROMK from 7.2 +/- 0.1 (n = 6) in 1 mM K to 6.8 +/- 0.01 (n = 4) in 1 mM K + 104 mM Cs. Surprisingly, the binding and washout of the specific blocker, Tertiapin-Q, also activated ROMK in 1 mM K and caused a comparable shift in apparent pK(a). These results are interpreted in terms of both a three-state kinetic model and a two-gate structural model that is based on results with KcsA in which the selectivity filter can assume either a high or low K conformation. In this context, external K, Cs, and Tertiapin-Q activate ROMK by destabilizing the low-K (collapsed) configuration of the selectivity filter.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.