ArticleBMC biotechnology2003
Disruption of vitellogenin gene function in adult honeybees by intra-abdominal injection of double-stranded RNA.
Article in BMC biotechnology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 100 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
100 citing papers in PubMed, 314 citations in OpenAlex.
- Honey bee nutritranscriptomics reveals key insights towards precision nutrition.Journal of advanced research · 2026Article
- Deformed wing virus affects foraging success and foraging specialization of honeybee workers.Scientific reports · 2025Article
- AI-enhanced marker-assisted selection concept for the multifunctional honey bee (Hymenoptera: Apidea) protein Vitellogenin (Vg).Journal of economic entomology · 2025Article
- Evidence for vitellogenin DNA-binding in honey bees.Protein science : a publication of the Protein Society · 2025Article
- Review
- Transcriptomic Analysis of Genes Associated with Stinger Development at Different Life Stages ofInternational journal of molecular sciences · 2024Article
- Honey bee functional genomics using symbiont-mediated RNAi.Nature protocols · 2023Review
- Circular RNA ame_circ_2015 Function as microRNA Sponges in Regulating Egg-Laying of Honeybees (Life (Basel, Switzerland) · 2023Article
- Silencing an aphid-specific geneFrontiers in plant science · 2022Article
- Molecular underpinnings of division of labour among workers in a socially complex termite.Scientific reports · 2021Article
- Societies to genes: can we get there from here?Genetics · 2021Article
- Organ-specific transcriptome analysis reveals differential gene expression in different castes under natural conditions in Apis cerana.Scientific reports · 2021Article
- Social modulation of ageing: mechanisms, ecology, evolution.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Review
- Article
- Article
- Article
- Article
- Insulin Receptor Substrate Gene Knockdown Accelerates Behavioural Maturation and Shortens Lifespan in Honeybee Workers.Insects · 2019Article
- Review
- Targeting the potassium ion channel genes SK and SH as a novel approach for control of insect pests: efficacy and biosafety.Pest management science · 2019Article
40 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe ability to manipulate the genetic networks underlying the physiological and behavioural repertoires of the adult honeybee worker (Apis mellifera) is likely to deepen our understanding of issues such as learning and memory generation, ageing, and the regulatory anatomy of social systems in proximate as well as evolutionary terms. Here we assess two methods for probing gene function by RNA interference (RNAi) in adult honeybees.
resultsThe vitellogenin gene was chosen as target because its expression is unlikely to have a phenotypic effect until the adult stage in bees. This allowed us to introduce dsRNA in preblastoderm eggs without affecting gene function during development. Of workers reared from eggs injected with dsRNA derived from a 504 bp stretch of the vitellogenin coding sequence, 15% had strongly reduced levels of vitellogenin mRNA. When dsRNA was introduced by intra-abdominal injection in newly emerged bees, almost all individuals (96%) showed the mutant phenotype. An RNA-fragment with an apparent size similar to the template dsRNA was still present in this group after 15 days.
conclusionInjection of dsRNA in eggs at the preblastoderm stage seems to allow disruption of gene function in all developmental stages. To dissect gene function in the adult stage, the intra-abdominal injection technique seems superior to egg injection as it gives a much higher penetrance, it is much simpler, and it makes it possible to address genes that are also expressed in the embryonic, larval or pupal stages.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.