ArticleTransactions of the American Ophthalmological Society2002
Diabetes-induced dysfunction of retinal Müller cells.
Article in Transactions of the American Ophthalmological Society, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 90 citations in OpenAlex.
- Müller cell glutamine metabolism links photoreceptor and endothelial injury in diabetic retinopathy.Life science alliance · 2026Article
- Systemic and Ocular Predictive Factors in the Treatment of Diabetic Macular Edema with Bevacizumab.Medicina (Kaunas, Lithuania) · 2026Article
- Intravenous delivery of mesenchymal stromal cells reverses Müller cell endoplasmic reticulum stress in diabetic retinopathy.Stem cell research & therapy · 2025Article
- Sustained Benefit of Short-Term Levodopa Treatment on Inner Retinal Function in Patients With Diabetes.Translational vision science & technology · 2025Article
- Article
- Biochemical mechanism underlying the pathogenesis of diabetic retinopathy and other diabetic complications in humans: the methanol-formaldehyde-formic acid hypothesis.Acta biochimica et biophysica Sinica · 2022Article
- Pharmacological Inhibition of Spermine Oxidase Suppresses Excitotoxicity Induced Neuroinflammation in Mouse Retina.International journal of molecular sciences · 2022Article
- Time dependent effects of prolonged hyperglycemia in zebrafish brain and retina.Frontiers in ophthalmology · 2022Article
- Inhibiting the activation of MAPK (ERK1/2) in stressed Müller cells prevents photoreceptor degeneration.Theranostics · 2022Article
- The innate immune system in diabetic retinopathy.Progress in retinal and eye research · 2021Review
- Diabetic retinal neurodegeneration as a form of diabetic retinopathy.International ophthalmology · 2021Review
- Glycemic Gap as a Useful Surrogate Marker for Glucose Variability and Progression of Diabetic Retinopathy.Journal of personalized medicine · 2021Article
- PACAP and NAP: Effect of Two Functionally Related Peptides in Diabetic Retinopathy.Journal of molecular neuroscience : MN · 2021Review
- Photoreceptor cells and RPE contribute to the development of diabetic retinopathy.Progress in retinal and eye research · 2021Review
- Quantitative analysis of retinal vessel density and thickness changes in diabetes mellitus evaluated using optical coherence tomography angiography: a cross-sectional study.BMC ophthalmology · 2021Observational
- Dietary glutamic acid and aspartic acid as biomarkers for predicting diabetic retinopathy.Scientific reports · 2021Observational
- Diabetic photoreceptors: Mechanisms underlying changes in structure and function.Visual neuroscience · 2020Review
- Emerging Insights and Interventions for Diabetic Retinopathy.Current diabetes reports · 2019Review
- The Diurnal Rhythm of Insulin Receptor Substrate-1 (IRS-1) and Kir4.1 in Diabetes: Implications for a Clock Gene Bmal1.Investigative ophthalmology & visual science · 2019Article
- A Robust Model System for Retinal Hypoxia: Live Imaging of Calcium Dynamics and Gene Expression Studies in Primary Human Mixed Retinal Culture.Frontiers in neuroscience · 2019Article
Corrections and comments
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Authors and funding
1 author.
Funding
Abstract
purposeThis study tested the hypothesis that the function of the glutamate transporter in retinal Müller cells is compromised early in the course of diabetes by a mechanism involving oxidation. Dysfunction of this transporter, which removes glutamate from the extracellular space, may play a critical role in the disruption of glutamate homeostasis that occurs in the diabetic retina. Because glutamate is toxic to retinal neurons and is likely to exacerbate oxidative stress, elucidation of the mechanisms by which diabetes elevates the concentration of this amino acid may help to better understand the pathogenesis of diabetic retinopathy.
methodsMüller cells were freshly isolated from normal rats and those made diabetic by streptozotocin injection. The activity of the Müller cell glutamate transporter, which is electrogenic, was monitored via the perforated-patch configuration of the patch-clamp technique.
resultsFour weeks after the onset of hyperglycemia, dysfunction of the Müller cell glutamate transporter was detected (P = .005). After 13 weeks of streptozotocin-induced diabetes, the activity of this transporter was decreased by 67% (P = .001). Consistent with oxidation causing this dysfunction, exposure to a disulfide-reducing agent rapidly restored the activity of this transporter in Müller cells from diabetic retinas.
conclusionsSoon after the onset of experimental diabetes, the function of the glutamate transporter in Müller cells is decreased by a mechanism that is likely to involve oxidation. The demonstration that the activity of this transporter can be rapidly restored raises the possibility that targeting this molecule for therapeutic intervention may restore glutamate homeostasis and, thereby, ameliorate sight-threatening complications of diabetic retinopathy.
Indexed as
Identifiers
12545700PMC1358969W2149104543What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.