ArticleThe British journal of ophthalmology2003
Trypan blue staining of internal limiting membrane and epiretinal membrane during vitrectomy: visual results and histopathological findings.
Article in The British journal of ophthalmology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT00419185 (Internal Limiting Membrane), which is not on this map. Cited by 43 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Internal Limiting Membrane (ILM)- Peeling With Indocyanine Green or Trypan Blue in Macular Hole Surgery: A Prospective Randomized Trial
Who cites it
43 citing papers in PubMed, 1 synthesis or guideline pooled it, 194 citations in OpenAlex.
- Vitrectomy for idiopathic macular hole.The Cochrane database of systematic reviews · 2015Pooled it
- Comparison of macular function and visual fields after membrane blue or infracyanine green staining in vitreoretinal surgery.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2010Trial
- Pharmacological adjuvants for diabetic vitrectomy surgery.World journal of methodology · 2024Review
- [Selective vital dyes in macular surgery : Do they increase the probability of intraoperative identification of the internal limiting membrane also for an experienced surgeon?]Die Ophthalmologie · 2024Article
- The Role of the Intraoperative Optical Coherence Tomography for Vitreoretinal Surgery in a Real-Life Setting.Life (Basel, Switzerland) · 2023Article
- Internal limiting membrane flap technique in macular hole surgery.International journal of ophthalmology · 2020Review
- [Heavy liquids as intraoperative instrument in retinal surgery].Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft · 2019Review
- Long-term results of surgical excision of conjunctival retention cyst using trypan blue with methylcellulose.American journal of ophthalmology case reports · 2019Article
- Modified technique of internal limiting membrane staining in idiopathic macular hole surgery.International ophthalmology · 2019Article
- Three-Dimensional Analysis of Peeled Internal Limiting Membrane Using Focused Ion Beam/Scanning Electron Microscopy.Translational vision science & technology · 2018Article
- Photochemical degradation of trypan blue.PloS one · 2018Article
- Clinical utility of intraoperative optical coherence tomography.Current opinion in ophthalmology · 2016Review
- Macular Hole Surgery with Internal Limiting Membrane Peeling Facilitated by Membrane-Blue® versus Membrane-Blue-Dual®: A Retrospective Comparative Study.Journal of ophthalmology · 2016Article
- Is it necessary to cover the macular hole with the inverted internal limiting membrane flap in macular hole surgery? A case report.BMC ophthalmology · 2015Article
- A review of current management of vitreomacular traction and macular hole.Journal of ophthalmology · 2015Review
- Complications of Macular Peeling.Journal of ophthalmology · 2015Review
- Chromovitrectomy: an update.Journal of ophthalmic & vision research · 2014Review
- Article
- Chromovitrectomy: update.Saudi journal of ophthalmology : official journal of the Saudi Ophthalmological Society · 2013Review
- Article
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimsTo report on the use of trypan blue (TB) 0.06% for staining the internal limiting membrane (ILM) and epiretinal membrane (ERM) during vitrectomy and report on their histology.
method14 consecutive patients with idiopathic macular hole or macular pucker (seven patients each) were prospectively recruited for ILM or ERM peel respectively. After pars plana vitrectomy and induction of posterior vitreous detachment, 0.5 ml TB 0.06% in phosphate buffered saline (VisonBlue) was injected over the posterior pole in an air filled eye and left for 2 minutes. The stained tissue was peeled with intraocular forceps. Specimens were evaluated using histochemical and immunohistochemical methods.
resultsThe average follow up was 4.4 months. Internal limiting membranes and epiretinal membranes were stained satisfactorily in all cases and removed successfully. Eight patients (57%) had improvement of 2 or more Snellen lines. All seven macular holes closed. In the ERM cases, no residual membranes were observed clinically, at the latest follow up. No complications relating to the use of the dye were encountered intraoperatively or postoperatively. Of the 14 procedures, nine (four macular hole and five macular pucker) yielded sufficient tissue for histopathological evaluation. Histological and immunohistological assessment revealed that the morphology of these specimens was similar to that observed in macular hole ILM and macular pucker ERM removed without the aid of dye.
conclusionTB staining facilitated the identification and delineation of ILM and ERM removal during the surgical management of macular holes and macular pucker. The visual outcome of this series and the specimens removed suggest they are no different from those without TB staining. Its use in posterior segment appears to be safe but further studies are required to investigate its long term safety.
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