Evidence map›Paper›PMID 12539581›Full record

ArticleJournal of Tongji Medical University = Tong ji yi ke da xue xue bao2001

Anti-viral activity of hairpin ribozyme directed against HBV core region in vitro.

J Lin, Y Song, X Kong, N Xie, X Wu, N Liu, N Wang, E Cao, Y Jin

Abstract read
PubMed Publisher
In one paragraph

Article in Journal of Tongji Medical University = Tong ji yi ke da xue xue bao, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 96% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 2 countries.

J LinInstitute of Liver Diseases, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
Y Song
X Kong
N Xie
X Wu
N Liu
N Wang
E Cao
Y Jin
Huazhong University of Science and Technology · CNTongji Hospital · CNMRC Laboratory of Molecular Biology · GBState Key Laboratory of Molecular Biology

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

To study the preparation and cleavage of hairpin ribozyme (HpRz) directed against the transcript of HBV core region in vitro, HRz gene designed by computer targeting the transcript of HBV core gene was cloned into the vector p1.5 between 5'-cis-Rz and 3'-cis-Rz. 32P-labeled HpRz transcript proved whether the vector fit for the preparation of hairpin ribozyme. 32P-labeled pKC transcript containing HBV core region as targets-RNA was transcribed by using T7 RNA polymerase and purified by PAGE. Cold HpRz transcript was incubated with 32P-labeled target-RNAs under different conditions and radioautographed after denaturing polyacrylamide gel electrophoresis. The results showed that HpRz had the ability of cleavage at 37 degrees C and 12 mmol/L MgCl2 and the design of ribozyme was correct. It is concluded that HpRz prepared in vitro possesses specific catalytic activity, indicating that it is possible for HpRz to intracellularly inhibit the replication of HBV. It may be developed into a nucleic acid drug in the treatment of hepatitis B in the future.

Indexed as

Antiviral AgentsBase SequenceEnzyme ActivationEscherichia coliGene Transfer TechniquesHepatitis B Core AntigensHepatitis B virusMolecular Sequence DataRNA, CatalyticRNA, ViralTranscription, GeneticVirus ReplicationAntiviral Agentshairpin ribozymeHepatitis B Core AntigensRNA, CatalyticRNA, Viral

Identifiers

PMID12539581
OpenAlexW1554275393

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.