ArticleProceedings of the National Academy of Sciences of the United States of America2003
Without peripheral interference, thymic deletion is mediated in a cohort of double-positive cells without classical activation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed, 31 citations in OpenAlex.
- Modulation of TCR signalling components occurs prior to positive selection and lineage commitment in iNKT cells.Scientific reports · 2021Article
- Elimination of self-reactive T cells in the thymus: a timeline for negative selection.PLoS biology · 2013Article
- Examination of thymic positive and negative selection by flow cytometry.Journal of visualized experiments : JoVE · 2012Article
- The role of the thymus in tolerance.Transplantation · 2010Review
- Central tolerance: what have we learned from mice?Seminars in immunopathology · 2008Review
- Clonal deletion of thymocytes can occur in the cortex with no involvement of the medulla.The Journal of experimental medicine · 2008Article
- Loss of c-Cbl RING finger function results in high-intensity TCR signaling and thymic deletion.The EMBO journal · 2005Article
- The timing of TCR alpha expression critically influences T cell development and selection.The Journal of experimental medicine · 2005Article
- Chronic inhaled ovalbumin exposure induces antigen-dependent but not antigen-specific inhalational tolerance in a murine model of allergic airway disease.The American journal of pathology · 2004Article
- Responses against complex antigens in various models of CD4 T-cell deficiency: surprises from an anti-CD4 antibody transgenic mouse.Immunologic research · 2004Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Peripheral activation can cause bystander thymocyte death by eliciting a "cytokine storm." This event complicates in vivo studies using exogenous ligand-induced models of negative selection. A stable transgenic model that selectively eliminates peripheral CD4 cells has allowed us to analyze negative selection as direct cognate events in two T cell receptor transgenic mice, OT-II and DO11. Whereas cognate peptide induced a massive deletion in double-positive (DP) cells in mice with peripheral CD4 cells, this DP deletion was modest in mice lacking peripheral CD4 cells. Using BrdUrd and annexin V staining, we found that negative selection primarily occurs in a cohort of DP cells and the absence of single-positive (SP) cells is largely caused by reduction in the cohort of DP precursors. Moreover, the fates of DP cells and SP cells after antigen exposure were vastly different. Whereas SP cells up-regulated uniformly their CD69 and CD44 levels, increased their cell size, and survived after antigen exposure, DP cells had less CD69 and CD44 up-regulation, no size change, and promptly died. Thus, negative selection represents an "abortive" activation different from activation-induced cell death of mature T cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.