ArticleProceedings of the National Academy of Sciences of the United States of America2003
Secretory granules are recaptured largely intact after stimulated exocytosis in cultured endocrine cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 154 papers.
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154 citing papers in PubMed, 360 citations in OpenAlex.
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- Vesicle docking and fusion pore modulation by the neuronal calcium sensor Synaptotagmin-1.Biophysical journal · 2025Article
- Comparison of localization and release of multivesicular bodies and secretory granules in islet cells: Dysregulation during type-2 diabetes.Journal of extracellular biology · 2024Article
- Vesicle docking and fusion pore modulation by the neuronal calcium sensor Synaptotagmin-1.bioRxiv : the preprint server for biology · 2024Article
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- Membrane transformations of fusion and budding.Nature communications · 2024Review
- Exosome secretion kinetics are controlled by temperature.Biophysical journal · 2023Article
- Novel genetically encoded tools for imaging or silencing neuropeptide release from presynaptic terminalsbioRxiv : the preprint server for biology · 2023Article
- Insulin secretion assays in an engineered MIN6 cell line.MethodsX · 2023Article
- Confocal Microscopy Studies of F-Actin Cytoskeleton Distribution and Dynamics Using Fluorescent LifeAct Constructs in Bovine Adrenal Chromaffin Cells.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Porosomes in uterine epithelial cells: Ultrastructural identification and characterization during early pregnancy.Journal of morphology · 2022Article
- Illuminating membrane structural dynamics of fusion and endocytosis with advanced light imaging techniques.Biochemical Society transactions · 2022Review
- Real-time visualization of exo- and endocytosis membrane dynamics with confocal and super-resolution microscopy.STAR protocols · 2022Article
- Sequential compound fusion and kiss-and-run mediate exo- and endocytosis in excitable cells.Science advances · 2022Article
- Rapid propagation of membrane tension at retinal bipolar neuron presynaptic terminals.Science advances · 2022Article
- Dynamin deficiency causes insulin secretion failure and hyperglycemia.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- The nanoscale molecular morphology of docked exocytic dense-core vesicles in neuroendocrine cells.Nature communications · 2021Article
- The structure and spontaneous curvature of clathrin lattices at the plasma membrane.Developmental cell · 2021Article
94 more citing papers are in PubMed but not listed here.
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5 authors at 1 institution in 2 countries.
Funding
Abstract
Classical cell biology teaches that exocytosis causes the membrane of exocytic vesicles to disperse into the cell surface and that a cell must later retrieve by molecular sorting whatever membrane components it wishes to keep inside. We have tested whether this view applies to secretory granules in intact PC-12 cells. Three granule proteins were labeled with fluorescent proteins in different colors, and two-color evanescent-field microscopy was used to view single granules during and after exocytosis. Whereas neuro-peptide Y was lost from granules in seconds, tissue plasminogen activator (tPA) and the membrane protein phogrin remained at the granule site for over 1 min, thus providing markers for postexocytic granules. When tPA was imaged simultaneously with cyan fluorescent protein (CFP) as a cytosolic marker, the volume occupied by the granule appeared as a dark spot where it excluded CFP. The spot remained even after tPA reported exocytosis, indicating that granules failed to flatten into the cell surface. Phogrin was labeled with GFP at its luminal end and used to sense the pH in granules. When exocytosis caused the acidic granule interior to neutralize, GFP-phogrin at first brightened and later dimmed again as the interior separated from the extracellular space and reacidified. Reacidification and dimming could be reversed by application of NH(4)Cl. We conclude that most granules reseal in <10 s after releasing cargo, and that these empty or partially empty granules are recaptured otherwise intact.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.