ArticleGenome biology2003
A gene-expression program reflecting the innate immune response of cultured intestinal epithelial cells to infection by Listeria monocytogenes.
Article in Genome biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 49 citations in OpenAlex.
- Transcriptome responses of intestinal epithelial cells induced by membrane vesicles ofCurrent research in microbial sciences · 2023Article
- Pathogenic Biohacking: Induction, Modulation and Subversion of Host Transcriptional Responses byToxins · 2020Review
- Rainbow Trout (Frontiers in immunology · 2019Article
- Listeria Adhesion Protein Induces Intestinal Epithelial Barrier Dysfunction for Bacterial Translocation.Cell host & microbe · 2018Article
- Missing value imputation for microarray data: a comprehensive comparison study and a web tool.BMC systems biology · 2013Article
- Temporal ordering of cancer microarray data through a reinforcement learning based approach.PloS one · 2013Article
- Illuminating the landscape of host-pathogen interactions with the bacterium Listeria monocytogenes.Proceedings of the National Academy of Sciences of the United States of America · 2011Review
- Early gene response of human brain microvascular endothelial cells to Listeria monocytogenes infection.Canadian journal of microbiology · 2011Article
- Phenotypic pattern-based assay for dynamically monitoring host cellular responses to Salmonella infections.PloS one · 2011Article
- The Listeria monocytogenes InlC protein interferes with innate immune responses by targeting the I{kappa}B kinase subunit IKK{alpha}.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Epithelial NF-kappaB enhances transmucosal fluid movement by altering tight junction protein composition after T cell activation.The American journal of pathology · 2010Article
- Upregulation of Salmonella-induced IL-6 production in Caco-2 cells by PJ-34, PARP-1 inhibitor: involvement of PI3K, p38 MAPK, ERK, JNK, and NF-kappaB.Mediators of inflammation · 2009Article
- Epithelial Cell Gene Expression Induced by Intracellular Staphylococcus aureus.International journal of microbiology · 2009Article
- Which missing value imputation method to use in expression profiles: a comparative study and two selection schemes.BMC bioinformatics · 2008Article
- Systems biology approach for mapping the response of human urothelial cells to infection by Enterococcus faecalis.BMC bioinformatics · 2007Article
- Gene expression programs in response to hypoxia: cell type specificity and prognostic significance in human cancers.PLoS medicine · 2006Article
- A prfA transposon mutant of Listeria monocytogenes F2365, a serotype 4b strain, is able to survive in the gastrointestinal tract but does not cause systemic infection of the spleens and livers of intragastrically inoculated mice.Infection and immunity · 2005Article
- Listeriolysin O-induced membrane permeation mediates persistent interleukin-6 production in Caco-2 cells during Listeria monocytogenes infection in vitro.Infection and immunity · 2005Article
- Murine macrophage transcriptional responses to Bacillus anthracis infection and intoxication.Infection and immunity · 2005Article
- A specific gene expression program triggered by Gram-positive bacteria in the cytosol.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundListeria monocytogenes is a Gram-positive, facultative, intracellular bacterial pathogen found in soil, which occasionally causes serious food-borne disease in humans. The outcome of an infection is dependent on the state of the infected individual's immune system, neutrophils being key players in clearing the microorganism from the body. The first line of host defense, however, is the intestinal epithelium.
resultsWe have examined the transcriptional response of cultured human intestinal epithelial cells to infection by L. monocytogenes, which replicates in the host cell cytoplasm and spreads from cell to cell using a form of actin-based motility. We found that the predominant host response to infection was mediated by NFkappaB. To determine whether any host responses were due to recognition of specific virulence factors during infection, we also examined the transcriptional response to two bacterial mutants; actA which is defective in actin-based motility, and prfA, which is defective in the expression of all L. monocytogenes virulence genes. Remarkably, we found no detectable difference in the host transcriptional response to the wild-type and mutant bacteria.
conclusionsThese results suggest that cultured intestinal epithelial cells are capable of mounting and recruiting a powerful innate immune response to L. monocytogenes infection. Our results imply that L. monocytogenes is not specifically detected in the host cytoplasm of Caco-2 cells by intracellular signals. This suggests that entry of bacteria is mediated in the host cell post-translationally, and that these bacteria seek the cytosol not only for the nutrient-rich environment, but also for protection from detection by the immune system.
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